The Nuclear Mitotic Apparatus (NuMA) Protein: A Key Player for Nuclear Formation, Spindle Assembly, and Spindle Positioning.
The Nuclear Mitotic Apparatus (NuMA) Protein: A Key Player for Nuclear Formation, Spindle Assembly, and Spindle Positioning.
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DOI：
10.3389/fcell.2021.653801
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发表时间：
2021
影响因子：
5.5
通讯作者：
Boerner S
中科院分区：
文献类型：
--
作者：
Kiyomitsu T;Boerner S
核有丝分裂器 (NuMA) 蛋白在脊椎动物中高度保守，并动态改变其亚细胞定位，从间期核到有丝分裂/减数分裂纺锤体极和有丝分裂细胞皮层。在这些位置，NuMA 分别充当核形成、纺锤体组装和有丝分裂纺锤体定位的关键结构枢纽。为了实现其可变功能，NuMA 与多种因素相互作用，包括 DNA、微管、质膜、输入蛋白和细胞质动力蛋白。 NuMA 通过其 N 端结构域与动力蛋白的结合驱动纺锤体极聚焦和纺锤体定位，而通过其 C 端区域的多重相互作用定义了其亚细胞定位和功能。此外，NuMA 可以自组装成高阶结构，这可能有助于纺锤体定位和核形成。在这篇综述中，我们总结了 NuMA 领域、功能和调控的最新进展，重点关注人类 NuMA，以了解脊椎动物 NuMA 与无脊椎动物 NuMA 相关蛋白相比如何以及为何参与这些功能。
The nuclear mitotic apparatus (NuMA) protein is well conserved in vertebrates, and dynamically changes its subcellular localization from the interphase nucleus to the mitotic/meiotic spindle poles and the mitotic cell cortex. At these locations, NuMA acts as a key structural hub in nuclear formation, spindle assembly, and mitotic spindle positioning, respectively. To achieve its variable functions, NuMA interacts with multiple factors, including DNA, microtubules, the plasma membrane, importins, and cytoplasmic dynein. The binding of NuMA to dynein via its N-terminal domain drives spindle pole focusing and spindle positioning, while multiple interactions through its C-terminal region define its subcellular localizations and functions. In addition, NuMA can self-assemble into high-ordered structures which likely contribute to spindle positioning and nuclear formation. In this review, we summarize recent advances in NuMA’s domains, functions and regulations, with a focus on human NuMA, to understand how and why vertebrate NuMA participates in these functions in comparison with invertebrate NuMA-related proteins.
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影响因子：
16.6
作者：
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通讯作者：
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DOI：
10.1083/jcb.201705168
发表时间：
2017-11-06
期刊：
The Journal of cell biology
影响因子：
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发表时间：
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期刊：
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作者：
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DOI：
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发表时间：
1992-03
期刊：
The Journal of cell biology
影响因子：
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影响因子：
7.7
作者：
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通讯作者：
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