Peptide nanofiber hydrogel adjuvanted live virus vaccine enhances cross-protective immunity to porcine reproductive and respiratory syndrome virus.
Peptide nanofiber hydrogel adjuvanted live virus vaccine enhances cross-protective immunity to porcine reproductive and respiratory syndrome virus.
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DOI：
10.1016/j.vaccine.2013.07.080
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发表时间：
2013-09-23
期刊：
影响因子：
5.5
通讯作者：
Shi, Jishu
中科院分区：
文献类型：
--
作者：
Li, Xiangdong;Galliher-Beckley, Amy;Huang, Hongzhou;Sun, Xiuzhi;Shi, Jishu
猪繁殖与呼吸综合征病毒（Porcine Reproductive and Respiratory Syndrome Virus，PRRSV）是世界范围内流行于养猪场的一种病毒，是造成经济损失和动物痛苦的主要原因。PRRSV的快速遗传变异使得目前的疫苗难以针对新出现的毒株提供保护。我们最近证明了一种新的肽水凝胶（H9 e）可以作为灭活H1N1疫苗的有效佐剂。因此，本研究的目的是评估H9 e作为PRRSV改良活病毒（MLV）疫苗的佐剂。在用VR-2332（亲本疫苗株）或MN 184 A（遗传多样性株）PRRSV攻毒之前，用含或不含H9 e佐剂的Ingelvac PRRSV MLV接种猪。接种MLV+ H9 e的猪具有更高水平的循环疫苗病毒。更重要的是，与单独接种MLV的猪相比，接种MLV+ H9 e的猪对两种PRRSV毒株攻击的保护性提高，如攻击诱导的病毒血症减少所证明的。与单独接种MLV的猪相比，接种MLV+ H9 e的猪具有较低的T调节细胞和IL-10产生频率，但Th/记忆细胞和IFN-γ分泌频率较高。综上所述，我们的研究表明，当与PRRSV MLV疫苗组合时，肽水凝胶H9 e可以通过调节宿主体液和细胞免疫应答来增强针对两种不同PRRSV毒株的疫苗效力。
Porcine reproductive and respiratory syndrome virus (PRRSV) is prevalent in swine farms worldwide and is a major source of economic loss and animal suffering. Rapid genetic variation of PRRSV makes it difficult for current vaccines to confer protection against newly emerging strains. We recently demonstrated that a novel peptide nanofiber hydrogel (H9e) could act as a potent adjuvant for killed H1N1 vaccines. Therefore, the objective of this study was to evaluate H9e as an adjuvant for PRRSV modified live virus (MLV) vaccines. Pigs were vaccinated with Ingelvac PRRSV MLV with or without H9e adjuvant before being challenged with the VR-2332 (parental vaccine strain) or MN184A (genetically diverse strain) PRRSV. Pigs vaccinated with MLV+H9e had higher levels of circulating vaccine virus. More importantly, pigs vaccinated with MLV+H9e had improved protection against challenge by both PRRSV strains, as demonstrated by reduced challenge-induced viremia compared with pigs vaccinated with MLV alone. Pigs vaccinated with MLV+H9e had lower frequency of T-regulatory cells and IL-10 production but higher frequency of Th/memory cells and IFN-γ secretion than that in pigs vaccinated with MLV alone. Taken together, our studies suggest that the peptide nanofiber hydrogel H9e, when combined with the PRRSV MLV vaccine, can enhance vaccine efficacy against two different PRRSV strains by modulating both host humoral and cellular immune responses.
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