Cells expressing Desiccate are essential for morphogenesis of labial sensilla in Drosophila melanogaster adults
Cells expressing Desiccate are essential for morphogenesis of labial sensilla in Drosophila melanogaster adults
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表达干燥的细胞对于黑腹果蝇成虫唇部感器的形态发生至关重要
DOI：
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Y. Hayakawa
中科院分区：
文献类型：
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作者：
Takeshi Kawano;H. Matsumoto;Emi Maekawa;F. Nakano;H. Kanuka;S. Tsuzuki;Y. Hayakawa
我们最近发现了一个新的基因，Desiccate（Desi），它在表皮中表达，并保护果蝇幼虫免受干燥胁迫。在本研究中，我们发现，味觉器官表达更多的Desi比表皮无论是在幼虫和成虫。在含有Desi的1，010-bp 5′侧翼区域的Desi启动子-Gal 4系的指导下，幼虫中的绿色荧光蛋白（GFP）表达在表皮中没有产生信号，但在幼虫味觉感觉器官的细胞中产生了强烈的信号，表明这种驱动程序专门在味觉器官中起作用。在成虫中，在唇瓣、跗骨和翅上的感受器的基底细胞中也观察到GFP表达。更精确的形态学分析的GFP表达位于其表达的外部辅助细胞，而不是神经元的唇感器。尽管Desi敲除或在Desi表达细胞中诱导细胞死亡不会改变幼虫的形态或生理特征，但缺乏Desi表达细胞的幼虫无法正常变形，并且所有幼虫都在蛹内死亡。死亡的pharate成人被发现缺乏所有唇感器。原神经基因Achaete和Scute参与成人中枢和周围神经系统的发育，在缺乏Desi表达细胞的puerum中正常表达。这些结果表明，Desi表达细胞的缺乏使得不可能产生用于感器发育的外部辅助细胞，从而表明表达Desi的细胞对于果蝇成虫唇感器的正常形态发生是必不可少的。
We recently discovered a new gene, Desiccate (Desi), that is expressed in the epidermis and protects larvae from desiccation stress in Drosophila melanogaster. In the present study, we found that taste organs express more Desi than the epidermis both in larvae and adults. Green fluorescent protein (GFP) expression in larvae under the direction of a Desi promoter‐Gal4 line containing the 1,010‐bp 5′ flanking region of Desi produced no signal in the epidermis but strong signals in cells of the larval gustatory sense organs, indicating that this driver works specifically in the gustatory organs. In adults, GFP expression was also observed in basal cells of sensilla on labella, tarsi and wings. More precise morphological analysis of GFP expression located its expression in the outer accessory cells rather than neurons of the labial sensilla. Although Desi knockdown or induction of cell death in Desi‐expressing cells did not change the morphological or physiological characters of the larvae, larvae lacking Desi‐expressing cells failed to metamorphose normally, and all of them died inside puparia. Dying pharate adults were found to lack all labial sensilla. The proneural genes Achaete and Scute, which are involved in the development of the adult central and peripheral nervous system, were normally expressed in the pupae lacking Desi‐expressing cells. These results suggested that the lack of Desi‐expressing cells makes it impossible to produce outer accessory cells for development of the sensilla, thereby signifying that cells expressing Desi are essential for normal morphogenesis of the labial sensilla in Drosophila adults.
影响因子：
10.5
作者：
S. Romani;S. Campuzano;E. Macagno;J. Modolell
通讯作者：
S. Romani;S. Campuzano;E. Macagno;J. Modolell
影响因子：
4.6
作者：
Hugo J. Bellen;D. D'evelyn;M. Harvey;Stephen J. Elledge
通讯作者：
Hugo J. Bellen;D. D'evelyn;M. Harvey;Stephen J. Elledge