Identification of the polyhydroxybutyrate granules in mammalian cultured cells.
Identification of the polyhydroxybutyrate granules in mammalian cultured cells.
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DOI：
10.1002/cbdv.201200294
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发表时间：
2012-11
影响因子：
2.9
通讯作者：
Pavlov, Evgeny
中科院分区：
文献类型：
--
作者：
Elustondo, Pia;Zakharian, Eleonora;Pavlov, Evgeny
聚-3-羟基丁酸酯（PHB）是存在于细菌和真核细胞中的生物聚酯。长链（或储存）sPHB（多达100，000个残基）通常存在于聚PHB细菌中，并定位于称为碳体的特殊颗粒中。在这些生物体中，sPHB作为碳和能量储存发挥着重要作用。另一方面，短链（或复合）cPHB（10-100个残基）存在于真核生物体中，包括哺乳动物以及许多细菌。先前的研究表明cPHB定位于真核生物的各种亚细胞区室。在这里，我们使用荧光显微镜直接研究哺乳动物细胞中的PHB的定位。使用荧光探针BODIPY 493/503在培养的U87细胞中观察到PHB。通过用PHB特异性解聚酶（PhaZ 7）处理的样品的荧光显著降低来证实PHB染色的特异性。我们发现，聚羟基丁酸与颗粒，这些聚羟基丁酸丰富的颗粒不共定位与线粒体，溶酶体，或内质网。这些结果表明，在哺乳动物细胞中，聚羟基丁酸可以积累在细胞质中的颗粒类似于“能量存储”的聚羟基丁酸积累细菌中发现的碳体。
Poly-3-hydroxybutyrate (PHB) is a biological polyester present in bacteria and eukaryotic cells. Long-chain (or storage) sPHB (up to 100,000 residues) is typically present in PHB-accumulating bacteria and localized in specialized granules known as carbonosomes. In these organisms, sPHB plays a major role as carbon and energy storage. On the other hand, short-chain (or complexed) cPHB (10–100 residues) is present in eukaryotic organisms, including mammals as well as in many bacteria. Previous studies indicated that cPHB is localized in various subcellular compartments of the eukaryotic organisms. Here, we used fluorescent microscopy to directly investigate the localization of PHB in mammalian cells. PHB was visualized in cultured U87 cells using fluorescent probe BODIPY 493/503. Specificity of PHB staining was confirmed by markedly decreased fluorescence of samples treated with PHB-specific depolymerase (PhaZ7). We found that PHB is associated with granules, and that these PHB-enriched granules do not co-localized with mitochondria, lysosomes, or endoplasmic reticulum. These results suggest that, in mammalian cells, PHB can accumulate in the cytoplasm in granules similar to ‘energy storage’ carbonosomes found in PHB-accumulating bacteria.
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影响因子：
2.1
作者：
Braaz, R;Handrick, R;Jendrossek, D
通讯作者：
Jendrossek, D
DOI：
10.1016/s0141-8130(99)00037-9
发表时间：
1999-06-01
影响因子：
8.2
作者：
Seebach, D;Fritz, MG
通讯作者：
Fritz, MG
影响因子：
4
作者：
Guo, Yi;Cordes, Kimberly R.;Walther, Tobias C.
通讯作者：
Walther, Tobias C.
DOI：
10.1073/pnas.85.12.4176
发表时间：
1988-06-01
影响因子：
11.1
作者：
REUSCH, RN;SADOFF, HL
通讯作者：
SADOFF, HL
影响因子：
3.4
作者：
Pavlov, E;Zakharian, E;French, RJ
通讯作者：
French, RJ