Post-natal changes in MCT1 expression in the forestomach of calves.
Post-natal changes in MCT1 expression in the forestomach of calves.
复制标题
犊牛前胃MCT1表达的产后变化
DOI：
10.1111/jpn.12057
复制
发表时间：
2013
影响因子：
2.7
通讯作者：
Gäbel
中科院分区：
文献类型：
--
作者：
Pfannkuche;Taifour;Steinhoff-Wagner;Hammon;Gäbel
单羧酸转运体1(MCT1)参与了短链脂肪酸(SCFA)和/或其上皮内代谢产物从瘤胃到血液的转运。由于MCT1在SCFA转移中起作用，推测SCFA是影响其表达的主要底物。然而，有迹象表明，当SCFA不在前胃中释放时，MCT1也可能在动物的早期生命中表达。为了弄清在此期间前胃MCT1的表达是否独立于SCFA的影响，我们用免疫组织化学方法研究了出生后早产和足月小牛的瘤胃、反刍房、瘤胃背侧囊、腹侧瘤胃和网状结构中MCT1的表达。用初乳或以牛奶为基础的配方日粮喂养小牛。在所有被测试的前胃隔室中都可以发现MCT1，甚至在早产小牛身上也是如此。出生后24小时，未成熟上皮中蛋白质主要定位于管腔侧。细胞在出生后4天内开始向血侧定向。在瘤胃上皮细胞中(但在所测试的其他前胃隔室中不存在)，MCT1向细胞血液侧的定向与出生后4天内总体表达率的增加相平行。由于乳酸水平在出生后立即非常高，乳酸依赖的底物诱导可能是潜在的机制。然而，由于一般不同的过程而导致的非特定变化也可能是原因。MCT1的早期上调和高血乳酸水平可能为上皮细胞提供了乳酸作为能量来源。
The monocarboxylate transporter 1 (MCT1) has been demonstrated to be involved in the transfer of short‐chain fatty acids (SCFA) and/or their intraepithelial metabolites from the rumen to the blood. As MCT1 plays a role in SCFA transfer, it is assumed that SCFA are the main substrates influencing its expression. However, there are hints that MCT1 may also be expressed during the early life of the animal when SCFA are not released in the forestomach. To figure out whether MCT1 expression in the forestomach is influenced independently of SCFA during that period, we studied post‐natal MCT1 expression immunohistochemically in the epithelia of omasum, atrium ruminis, saccus dorsalis ruminis, saccus ventralis ruminis and reticulum of calves born preterm and at term. The calves were nourished by colostrum or by milk‐based formula diet. MCT1 could be found in all the forestomach compartments tested, even in preterm calves. The protein was mainly oriented to the luminal side in the immature epithelium 24 h after birth. Orientation to the blood side of the cells developed during the first 4 days after birth. In the rumen epithelia (but not in the other forestomach compartments tested), orientation of MCT1 to the blood side of the cells was paralleled by an increase in the overall expression rate during the first 4 days after birth. As lactate levels were very high directly after birth, a lactate‐dependent substrate induction may have been the underlying mechanism. However, non‐specific changes due to general differential processes might also be the cause. Both early upregulation of MCT1 and high blood lactate levels may provide the epithelia with lactate as energy source.
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羔羊瘤胃代谢和瘤胃上皮的发育。
DOI：
10.1080/17450399309386072
发表时间：
1993
期刊：
Archiv fur Tierernahrung
影响因子：
--
作者：
R. Žitňan;A. Bomba;A. Sommer;L. Kolodzieyski
通讯作者：
L. Kolodzieyski
DOI：
10.1152/ajpendo.2002.282.1.e154
发表时间：
2002-01-01
影响因子：
5.1
作者：
Green, H;Halestrap, A;Ouyang, J
通讯作者：
Ouyang, J
DOI：
10.1152/ajpgi.00268.2001
发表时间：
2002-11-01
影响因子：
4.5
作者：
Müller, F;Huber, K;Gäbel, G
通讯作者：
Gäbel, G
早产和足月犊牛内源性葡萄糖产生的成熟。
影响因子：
3.5
作者：
J. Steinhoff;S. Görs;P. Junghans;R. Bruckmaier;E. Kanitz;C. Metges;H. Hammon
通讯作者：
H. Hammon
年龄和精料喂养对山羊和赫里福德育肥公牛胃肠道 MCT 1 和 CD147 表达的影响。
影响因子：
2.4
作者：
N. Koho;J. Taponen;H. Tiihonen;M. Manninen;A. Pösö
通讯作者：
A. Pösö