Whole-transcriptome analysis of periodontal tissue and construction of immune-related competitive endogenous RNA network.
Whole-transcriptome analysis of periodontal tissue and construction of immune-related competitive endogenous RNA network.
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牙周组织全转录组分析及免疫相关竞争性内源RNA网络构建
DOI：
10.1186/s12903-022-02401-0
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发表时间：
2022-08-31
期刊：
影响因子：
2.9
通讯作者：
Lou, Xinzhe
中科院分区：
文献类型：
--
作者：
Zhao, Quanquan;Wen, Jing;Ouyang, Xiangying;Liu, Jianru;Liu, Wenyi;Zhang, Shengnan;Lv, Peiying;Lou, Xinzhe
在牙周炎中，非编码RNA可能通过竞争性内源性RNA在免疫微环境中发挥调节作用。我们的目的是分析牙周炎中非编码RNA的表达，并构建免疫相关的ceRNA网络。收集了五个发炎的牙周组织和五个健康的牙龈组织进行全转录组测序。通过差异基因、功能富集和蛋白质相互作用网络分析，探讨差异表达基因的功能。采用CIBERSORTx检测牙周组织中免疫细胞浸润水平。构建了一个与免疫相关的竞争性内源RNA网络，并验证了网络中关键调控因子的表达。 与健康牙龈相比，200个mRNA、90个长链非编码RNA、65个microRNA和518个环状RNA在炎症牙周组织中差异表达，细胞趋化性显著增强。免疫细胞浸润分析显示，中性粒细胞，巨噬细胞M1，T滤泡辅助细胞和幼稚B细胞在牙周炎中显著增加。鉴定了关键调控因子包括JUN、FOS、THBS 1、KLF 2、WIF 1，然后验证了它们的表达。 我们构建了牙周组织中与免疫相关的竞争性内源RNA网络，为牙周炎的免疫稳态提供了新的见解，并为进一步研究非编码RNA奠定了基础。该网络中的关键调节因子可能是未来牙周炎治疗的有希望的靶点。在线版本包含补充材料，可通过10.1186/s12903-022-02401-0获得。
In periodontitis, noncoding RNAs may play a regulatory role in the immune microenvironment through competitive endogenous RNA. We aimed to profile noncoding RNA expression and construct immune-related ceRNA network in periodontitis. Five inflamed periodontal tissue and five healthy gingivae were collected for whole-transcriptome sequencing. Differential gene, functional enrichment, and protein–protein interaction network analysis were performed to explore the function of differentially expressed genes. CIBERSORTx was used to analyze level of immune cell infiltration in the periodontal tissue. An immune-related competitive endogenous RNA network was constructed and expression of key regulators in the network was validated. Compared with healthy gingiva, 200 mRNAs, 90 long noncoding RNAs, 65 microRNAs, and 518 circular RNAs were differentially expressed, and cell chemotaxis was significantly enhanced in inflamed periodontal tissue. Immune cell infiltration analysis showed that neutrophils, macrophages M1, T follicular helper cells, and naive B cells were significantly increased in periodontitis. Key regulators including JUN, FOS, THBS1, KLF2, WIF1, were identified and their expression was then validated. We constructed an immune-related competitive endogenous RNA network in periodontal tissue, which provided new insights into immune homeostasis in periodontitis and laid a foundation for further study of noncoding RNAs. Key regulators in this network may be promising targets for future periodontitis treatment. The online version contains supplementary material available at 10.1186/s12903-022-02401-0.
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影响因子：
3.7
作者：
Ning W;Acharya A;Sun Z;Ogbuehi AC;Li C;Hua S;Ou Q;Zeng M;Liu X;Deng Y;Haak R;Ziebolz D;Schmalz G;Pelekos G;Wang Y;Hu X
通讯作者：
Hu X
DOI：
10.1165/ajrcmb.15.1.8679226
发表时间：
1996-07-01
影响因子：
6.4
作者：
Nicholson, WJ;Slight, J;Donaldson, K
通讯作者：
Donaldson, K
影响因子：
3.7
作者：
Schmidt J;Jentsch H;Stingu CS;Sack U
通讯作者：
Sack U
影响因子：
3.5
作者：
Matarese, Giovanni;Curro, Monica;Ientile, Riccardo
通讯作者：
Ientile, Riccardo
M1巨噬细胞通过旁分泌调节TLR4/AP1促进牙周炎牙槽骨破坏
影响因子：
3.8
作者：
Zhu, Li-Fang;Li, Lu;Xu, Yan
通讯作者：
Xu, Yan