General immune status and oral microbiology in patients with different forms of periodontitis and healthy control subjects.
General immune status and oral microbiology in patients with different forms of periodontitis and healthy control subjects.
复制标题
DOI：
10.1371/journal.pone.0109187
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Sack U
中科院分区：
文献类型：
--
作者：
Schmidt J;Jentsch H;Stingu CS;Sack U
免疫过程中的牙周炎的发病机制，特别是侵略性的形式，没有得到很好的理解。本研究探讨了健康对照组和不同形式的牙周炎患者的临床以及全身免疫学和局部微生物学特征。14名健康受试者，15名诊断为侵袭性牙周炎的患者，11名慢性牙周炎患者被招募。对每例患者进行牙周检查并采集外周血。流式细胞术和酶联免疫吸附斑点试验测定淋巴细胞群以及T辅助细胞释放的细胞因子。龈下菌斑样本从每个人，并立即培养微生物检查。用脂多糖刺激外周血单个核细胞（PBMCs）时，中度牙周炎患者IL-1β的释放量明显高于其他各组（p<0.01）。所有组的B细胞、幼稚和过渡B细胞、记忆B细胞和转换记忆B细胞的数量均在参考范围内，但慢性牙周炎患者的记忆B细胞百分比最高，无类别转换（p = 0.01）。 牙周炎患者龈下菌斑的数量和质量不同，革兰氏阴性厌氧菌的数量较多。齿垢普雷沃菌在侵袭性牙周炎患者中更常见（p<0.001），但与任何全身免疫学检查结果均无关联。牙龈卟啉单胞菌，这是只发现在中度慢性牙周炎患者，似乎与激活全身免疫反应。侵袭性牙周炎和中度慢性牙周炎之间的差异是显而易见的，这提出了侵袭性牙周炎中全身免疫反应和细菌感染之间不平衡的问题。
Immunological processes in the etiopathogenesis of periodontitis, especially the aggressive form, are not well understood. This study examined clinical as well as systemic immunological and local microbiological features in healthy controls and patients with different forms of periodontitis. 14 healthy subjects, 15 patients diagnosed with aggressive periodontitis, and 11 patients with chronic periodontitis were recruited. Periodontal examination was performed and peripheral blood was collected from each patient. Lymphocyte populations as well as the release of cytokines by T-helper cells were determined by flow cytometry and enzyme linked immunosorbent spot assay. Subgingival plaque samples were taken from each individual and immediately cultivated for microbiological examination. When stimulating peripheral blood mononuclear cells (PBMCs) with lipopolysaccharide, a higher IL-1β release was found in patients with moderate chronic periodontitis compared to the other groups (p<0.01). Numbers of B-cells, naïve and transitional B-cells, memory B-cells, and switched memory B-cells were within the reference range for all groups, but patients with chronic periodontitis showed the highest percentage of memory B-cells without class switch (p = 0.01). The subgingival plaque differed quantitatively as well as qualitatively with a higher number of Gram-negative anaerobic species in periodontitis patients. Prevotella denticola was found more often in patients with aggressive periodontitis (p<0.001) but did not show an association to any of the systemic immunological findings. Porphyromonas gingivalis, which was only found in patients with moderate chronic periodontitis, seems to be associated with an activation of the systemic immune response. Differences between aggressive periodontitis and moderate chronic periodontitis are evident, which raises the question of an inadequate balance between systemic immune response and bacterial infection in aggressive periodontitis.
登录
查看更多内容
影响因子：
3.1
作者：
Darveau, Richard P.
通讯作者：
Darveau, Richard P.
影响因子：
4.3
作者：
MARAZITA, ML;BURMEISTER, JA;SCHENKEIN, HA
通讯作者：
SCHENKEIN, HA
影响因子：
4.3
作者：
Becerik, Sema;Ozturk, Veil Ozgen;Emingil, Gulnur
通讯作者：
Emingil, Gulnur
影响因子：
6.7
作者：
Mombelli, A;Casagni, F;Madianos, PN
通讯作者：
Madianos, PN
影响因子：
3
作者：
Goncalves, Thais Oliveira;Costa, Dirceu;Nogueira-Filho, Getulio
通讯作者：
Nogueira-Filho, Getulio