β-Dispersion of blood during sedimentation.
β-Dispersion of blood during sedimentation.
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DOI：
10.1038/s41598-021-82171-x
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发表时间：
2021-01-29
影响因子：
4.6
通讯作者：
Aubry N
中科院分区：
文献类型：
--
作者：
Sabuncu AC;Muldur S;Cetin B;Usta OB;Aubry N
人类红细胞 (RBC) 的聚集是从细菌感染到癌症等各种病理状况的核心。当处于低剪切条件或止血时，红细胞会形成聚集体，类似于一堆硬币，称为“轮子”。我们通过实验检查了聚集红细胞的界面介电色散。 Hetastarch 是一种红细胞聚集剂，用于模拟导致聚集的条件。健康供体血液中 Hetastrach 的浓度逐渐增加，以测量该技术的灵敏度。当红细胞在 PDMS 室中形成肉卷和沉积物时，进行延时电阻抗测量。使用理论模型获得有效的单个红细胞聚集体在不同羟淀粉浓度下的复介电常数。还研究了红细胞阻抗的时间响应，以参数化阻抗数据的时间演变。聚集开始时的单聚集体介电常数、界面分散参数的演变以及沉降动力学使我们能够区分血液中的差异聚集。
Aggregation of human red blood cells (RBC) is central to various pathological conditions from bacterial infections to cancer. When left at low shear conditions or at hemostasis, RBCs form aggregates, which resemble stacks of coins, known as ‘rouleaux’. We experimentally examined the interfacial dielectric dispersion of aggregating RBCs. Hetastarch, an RBC aggregation agent, is used to mimic conditions leading to aggregation. Hetastrach concentration is incrementally increased in blood from healthy donors to measure the sensitivity of the technique. Time lapse electrical impedance measurements were conducted as red blood cells form rouleaux and sediment in a PDMS chamber. Theoretical modeling was used for obtaining complex permittivity of an effective single red blood cell aggregate at various concentrations of hetastarch. Time response of red blood cells’ impedance was also studied to parametrize the time evolution of impedance data. Single aggregate permittivity at the onset of aggregation, evolution of interfacial dispersion parameters, and sedimentation kinetics allowed us to distinguish differential aggregation in blood.
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影响因子：
3.4
作者：
ASAMI, K;TAKAHASHI, Y;TAKASHIMA, S
通讯作者：
TAKASHIMA, S
影响因子：
4.6
作者：
Dobbe, JGG;Streekstra, GJ;Grimbergen, CA
通讯作者：
Grimbergen, CA
影响因子：
2.5
作者：
Jung, CH;Park, SH;Kuhlman, MR
通讯作者：
Kuhlman, MR
影响因子：
2.1
作者：
Henkelman, Sandra;Rakhorst, Gerhard;Busscher, Henk J.
通讯作者：
Busscher, Henk J.
影响因子：
6.6
作者：
Koklu A;Sabuncu AC;Beskok A
通讯作者：
Beskok A