Distribution of hepatitis C virus genotypes in beta‐thalassaemic patients from Northern India
Distribution of hepatitis C virus genotypes in beta‐thalassaemic patients from Northern India
复制标题
印度北部β-地中海贫血患者丙型肝炎病毒基因型的分布
作者：
A. Chakravarti;V. Verma
多次输血的β -地中海贫血患者是丙型肝炎病毒（HCV）感染流行率较高的人群，因为其传播来自血清阴性窗口期收集的受感染血液。地中海贫血患者的HCV基因分型主要对患者的临床管理和促进治疗决策有用。因此，本研究的目的是确定地中海贫血患者中普遍存在的基因型，并将这些基因型与性别、年龄、输血次数和肝功能测试资料联系起来。逆转录聚合酶链反应（RT - PCR）对80例乙型肝炎病毒或人类免疫缺陷病毒抗体血清阴性的β -地中海贫血患者（男性58例，女性22例）进行了HCV RNA检测。采用Chinchai等人的限制性片段长度多态性（RFLP）方法进行HCV基因分型。型特异性PCR随后直接测序也被用于确认混合基因型感染。80例地中海贫血患者中，基因3型和基因1型分别感染20例和8例，基因1c/5a型感染2例。两组HCV感染和未感染的地中海贫血患者血清天冬氨酸转氨酶、丙氨酸转氨酶和碱性磷酸酶水平均有显著变化。基因型与性别、年龄、输血次数无显著相关性，但基因1型ALP水平显著高于基因3型。基因3型是β -地中海贫血患者的主要类型，约45%的患者感染混合型。因此，检测HCV RNA将有助于减少在血清阴性窗口期收集的HCV感染血液的传播，而确定基因型将有助于采取不同的治疗政策，以更好地管理地中海贫血患者。
summary Multitransfused beta‐thalassaemic patients constitute a population having a higher prevalence of hepatitis C virus (HCV) infection because of its transmission from infected blood collected during seronegative window period. HCV genotyping in thalassaemic patients is mainly useful for the clinical management of the patients and for facilitating decisions on therapy. Thus, the aim of the present study was to identify the genotypes that are prevalent in thalassaemic patients and to correlate these with gender, age, number of blood transfusions and the liver function test profiles. Reverse transcription–polymerase chain reaction (RT‐PCR) was carried out in 80 beta‐thalassaemic patients (58 men and 22 women) for detection of HCV RNA who were seronegative for hepatitis B virus or human immunodeficiency virus antibodies. HCV genotyping was carried out by restriction fragment length polymorphism (RFLP) method of Chinchai et al. Type‐specific PCR followed by direct sequencing was also used to confirm the mixed‐genotype infection. Among the 80 thalassaemic patients, 20 and eight patients were infected with genotypes 3 and 1, respectively, whereas two cases had infection with HCV genotype1c/5a. The serum levels of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase were found to be significantly altered between the two groups of HCV‐infected and noninfected thalassaemic patients. No significant correlation was observed between genotypes when compared with gender, age and number of blood transfusions, except significantly higher level of ALP in genotype 1 than in genotype 3. Genotype 3 alone was the predominant type in beta‐thalassaemic patients, with approximately 45% being infected with mixed type. Hence, detection of HCV RNA would help in decreasing the transmission of HCV‐infected blood collected during the seronegative window period, whereas determination of genotypes would provide help in adoption of different treatment policies for better management of thalassaemic patients.
影响因子：
158.5
作者：
McHutchison, JG;Gordon, SC;Albrecht, JK
通讯作者：
Albrecht, JK
影响因子：
6.4
作者：
Ray, SC;Arthur, RR;Thomas, DL
通讯作者：
Thomas, DL