Kinetic properties of Kv4.3 and their modulation by KChIP2b.
Kinetic properties of Kv4.3 and their modulation by KChIP2b.
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Kv4.3 的动力学特性及其 KChIP2b 的调节。
DOI：
10.1016/s0006-291x(02)00658-7
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发表时间：
2002
影响因子：
3.1
通讯作者：
Morales,MichaelJ
中科院分区：
文献类型：
--
作者：
Wang,Shimin;Patel,SangitaP;Qu,Yujie;Hua,Ping;Strauss,HaroldC;Morales,MichaelJ
KChIPs是Kv 4钾通道辅助亚基的一个家族，其作用通常包括减缓失活、加速从失活恢复以及增加通道表面表达。我们比较了270个氨基酸KChIP 2b对Kv4.3和Kv4.3内孔突变体[V（399，401）I]的作用。Kv4.3表现出快速失活，具有双指数时间过程，其中快速时间常数占主导地位。与野生型Kv4.3一起表达的KChIP 2b减慢了快速失活的时间常数;然而，由于缓慢失活阶段的贡献减少，整体失活速率更快。引入[V（399，401）I]使两个失活时间常数均减慢不到2倍。20 s脉冲持续时间后灭活不完全。KChIP 2b与Kv4.3 [V（399，401）I]的共表达显著减缓了失活。KChIP 2b使野生型通道的失活恢复率增加7.6倍，使Kv4.3 [V（399，401）I]的失活恢复率增加5.7倍。这些数据表明，内部孔结构是一个重要因素的KChIP 2b对Kv4.3 K+通道的调节作用。
KChIPs are a family of Kv4 K+channel ancillary subunits whose effects usually include slowing of inactivation, speeding of recovery from inactivation, and increasing channel surface expression. We compared the effects of the 270 amino acid KChIP2b on Kv4.3 and a Kv4.3 inner pore mutant [V(399, 401)I]. Kv4.3 showed fast inactivation with a bi-exponential time course in which the fast time constant predominated. KChIP2b expressed with wild-type Kv4.3 slowed the fast time constant of inactivation; however, the overall rate of inactivation was faster due to reduction of the contribution of the slow inactivation phase. Introduction of [V(399, 401)I] slowed both time constants of inactivation less than 2-fold. Inactivation was incomplete after 20s pulse durations. Co-expression of KChIP2b with Kv4.3 [V(399, 401)I] slowed inactivation dramatically. KChIP2b increased the rate of recovery from inactivation 7.6-fold in the wild-type channel and 5.7-fold in Kv4.3 [V(399,401)I]. These data suggest that inner pore structure is an important factor in the modulatory effects of KChIP2b on Kv4.3 K+channels.
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影响因子：
20.1
作者：
Dixon, JE;Shi, WM;McKinnon, D
通讯作者：
McKinnon, D
K( ) 通道相互作用蛋白 2 新型剪接变体的分子克隆和表达。
影响因子：
3.1
作者：
S. Ohya;Y. Morohashi;K. Muraki;T. Tomita;M. Watanabe;T. Iwatsubo;Y. Imaizumi
通讯作者：
Y. Imaizumi
Kv4.2电流与大鼠心脏瞬时外向电流之间的电生理和药理学对应关系。
影响因子：
10.8
作者：
Yeola,SW;Snyders,DJ
通讯作者：
Snyders,DJ
DOI：
10.1152/ajpcell.2001.281.1.c290
发表时间：
2001-07-01
影响因子：
5.5
作者：
Kuryshev, YA;Wible, BA;Brown, AM
通讯作者：
Brown, AM
DOI：
10.1152/ajpcell.2000.278.5.c931
发表时间：
2000-05-01
影响因子：
5.5
作者：
Kuryshev, YA;Gudz, TI;Wible, BA
通讯作者：
Wible, BA