The high mannose glycans from bovine ribonuclease B isomer characterization by ion trap MS.
The high mannose glycans from bovine ribonuclease B isomer characterization by ion trap MS.
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DOI：
10.1016/j.jasms.2008.11.012
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发表时间：
2009-04
影响因子：
3.2
通讯作者：
Reinhold VN
中科院分区：
文献类型：
--
作者：
Prien JM;Ashline DJ;Lapadula AJ;Zhang H;Reinhold VN
从牛核糖核酸酶B释放的三种糖聚体Man5GlcNAc2、Man7GlcNAc2和Man8GlcNAc2中已经发现了13种高甘露糖异构体的结构特征，其中6种以前未报道过。这项研究是用一个单一的离子阱仪器进行的，没有涉及色谱。从Man7和Man8中鉴定出三个先前表征的异构体（各三个），加上一个未报道的Man7异构体。Man6和Man7糖蛋白上不完全的α-葡萄糖苷酶活性似乎是另外两种异构体聚糖的原因。通过解析Man5糖蛋白中的三个新异构体，进一步证明了离子阱在详细分析中的重要性，这些异构体与该糖蛋白中先前未报道的六个结构相结合。所有报道的结构都代表了高尔基体加工残留物的分布，这些残留物属于Man9GlcNAc2足迹。拓扑结构是由离子组成途径定义的，而连接和分支是由一个小的低聚物片段库中的光谱同一性定义的。所描述的协议为揭示聚糖复杂性带来了多重优势（天线结构域选择性，同分异构体暴露，通过重整的产物光谱增强可检测性，有效的能量增强以获得更大的结构细节），这预示着该仪器是测序的理想选择。
Thirteen high mannose isomers have been structurally characterized within three glycomers, Man5GlcNAc2, Man7GlcNAc2, and Man8GlcNAc2 released from bovine ribonuclease B, six previously unreported. The study was carried out with a single ion trap instrument involving no chromatography. Three previously characterized isomers from Man7 and Man8 (three each) have been identified plus one unreported Man7 isomer. Incomplete α-glucosidase activity on the Man6 and Man7 glycoproteins appears to account for two additional isomeric glycans. The preeminence of ion traps for detail analysis was further demonstrated by resolving three new isomers within the Man5 glycomer summing to the six previously unreported structures in this glycoprotein. All reported structures represent a distribution of Golgi processing remnants that fall within the Man9GlcNAc2 footprint. Topologies were defined by ion composition pathways while linkage and branching by spectral identity in a small oligomer fragment library. The protocols described bring multiple advantages for unraveling glycan complexity, (antennal domain selectivity, isomer exposure, enhanced detectability with renormalized product spectra, effective energy enhancement for greater structural detail), that portend this instrument to be ideal for sequencing.
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DOI：
10.1016/j.jasms.2007.04.007
发表时间：
2007-07-01
影响因子：
3.2
作者：
Dwivedi, Prabha;Bendiak, Brad;Hill, Herbert H., Jr.
通讯作者：
Hill, Herbert H., Jr.
影响因子：
2.9
作者：
KAKEHI, K;SUZUKI, S;LEE, YC
通讯作者：
LEE, YC
影响因子：
4.3
作者：
LAINE, RA
通讯作者：
LAINE, RA
影响因子：
7.4
作者：
Lapadula, AJ;Hatcher, PJ;Reinhold, VN
通讯作者：
Reinhold, VN
影响因子：
3.1
作者：
FU, DT;CHEN, L;ONEILL, RA
通讯作者：
ONEILL, RA