Molecular collaborations between serpins and trefoil factor promote endodermal cell growth and gastrointestinal differentiation in budding tunicates
Molecular collaborations between serpins and trefoil factor promote endodermal cell growth and gastrointestinal differentiation in budding tunicates
复制标题
丝氨酸蛋白酶抑制剂和三叶因子之间的分子合作促进出芽被囊动物的内胚层细胞生长和胃肠道分化
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
S. Fujiwara
中科院分区：
文献类型：
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作者：
K. Kawamura;Yuko Kariya;Y. Ono;Akira Muramoto;K. Ohta;S. Fujiwara
我们提供的证据支持丝氨酸蛋白酶抑制剂（丝氨酸蛋白酶抑制剂）和三叶因子在被囊动物 Polyandrocarpa missakiensis 的出芽阶段之间的新合作。使用麦芽糖结合蛋白/P-丝氨酸蛋白酶抑制剂融合蛋白，从多雄果匀浆中提取出 40 kDa 和 45 kDa 的两种多肽。根据他们的部分氨基酸序列数据，克隆了一个 cDNA（928 bp）。它编码具有三叶共有基序的五个串联重复的多肽。因此，我们将 cDNA 命名为 P-trefoil。 P-三叶草和 P-丝氨酸蛋白酶抑制剂在出芽期间仅由体腔细胞表达。 P-Trefoil 在多雄果的无性生命周期中主要由体腔细胞表达，而 P-Serpin 特别定位于体腔细胞和发育芽的细胞外基质中。天然 P-Trefoil 蛋白表现出氨肽酶活性。它以 8 µg/mL 的浓度诱导培养的多雄果细胞的细胞生长。 P-丝氨酸蛋白酶抑制剂增强了 P-三叶草的这种活性。此外，P-Trefoil 和 P-Serpin 的混合物表现出体外诱导肠道特异性碱性磷酸酶的作用。这些结果首次表明丝氨酸蛋白酶抑制剂可以与三叶因子相互作用，在胃上皮的细胞生长和分化中发挥作用。
We present evidence supporting novel collaborations between the serine protease inhibitor (serpin) and the trefoil factor during the budding stage of the tunicate Polyandrocarpa misakiensis. Using a maltose‐binding protein/P‐serpin fusion protein, two polypeptides of 40 kDa and 45 kDa were pulled down from Polyandrocarpa homogenates. Based on their partial amino acid sequence data, a single cDNA (928 bp) was cloned. It encodes a polypeptide that has five tandem repeats of a trefoil consensus motif. Thus, we termed the cDNA P‐trefoil. Both P‐trefoil and P‐serpin were expressed exclusively by coelomic cells during budding. P‐Trefoil was expressed mainly by coelomic cells throughout the asexual life cycle of Polyandrocarpa, while P‐Serpin was localized particularly in coelomic cells and in the extracellular matrix in developing buds. The native P‐Trefoil protein showed aminopeptidase activity. It induced cell growth in cultured Polyandrocarpa cells at a concentration of 8 µg/mL. P‐Serpin reinforced this activity of P‐Trefoil. Further, a mixture of P‐Trefoil and P‐Serpin exhibited the in vitro induction of a gut‐specific alkaline phosphatase. These results show for the first time that a serpin can interact with a trefoil factor to play a role in the cellular growth and differentiation of the gastric epithelium.
抗凝血酶 III 是一种丝氨酸蛋白酶抑制剂家族蛋白酶抑制剂，是猪房水中的主要肝素结合蛋白。
DOI：
10.1006/bbrc.2000.2728
发表时间：
2000
期刊：
Biochemical and biophysical research communications.
影响因子：
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作者：
Rao,PV;Allingham,RR;Herndon,LW;Epstein,DL
通讯作者：
Epstein,DL
影响因子：
29.4
作者：
KINDON, H;POTHOULAKIS, C;PODOLSKY, DK
通讯作者：
PODOLSKY, DK