A Functional Kinase Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in Arabidopsis.
A Functional Kinase Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in Arabidopsis.
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DOI：
10.3389/fpls.2020.586870
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发表时间：
2020
影响因子：
5.6
通讯作者：
Doonan JH
中科院分区：
文献类型：
--
作者：
Nibau C;Dadarou D;Kargios N;Mallioura A;Fernandez-Fuentes N;Cavallari N;Doonan JH
在多变的环境中保持生育能力是开花植物繁殖成功的关键。减数分裂和花粉形成对生长条件的变化特别敏感，尤其是温度。我们先前已经发现细胞周期蛋白依赖的蛋白依赖性激酶G1(CDKG1)是温度依赖性减数分裂的主要调节因子，这可能涉及选择性剪接(AS)的调节，包括其自身的转录本。CDKG1mRNA可以经历几个AS事件，可能产生两个蛋白质变体：CDKG1L和CDKG1S，它们的N-末端结构域不同，可能参与协同因子的相互作用。在叶片中，这两种异构体对目标mRNA的加工具有不同的温度依赖功能，但它们在花粉发育中的作用尚不清楚。在本研究中，我们研究了CDKG1L和CDKG1S在维持拟南芥育性中的作用。我们发现，长(L)型是挽救cdkg1-1突变体育性缺陷的必要条件和充分条件，而短(S)型不能挽救育性缺陷。另一方面，CDKG1L的额外拷贝会降低生育能力。此外，该激酶的ATP结合口袋的突变表明，该激酶的活性是CDKG1功能所必需的。CDKG1L和CDKG1S的激酶突变体分别正确地定位于细胞核和胞核和细胞质，但不能挽救cdkg1-1突变体的生育或剪接缺陷。此外，我们还发现CDKG1和其类似的CDKG2之间存在部分功能重叠，这可以部分地用重叠的基因表达来解释。
Maintaining fertility in a fluctuating environment is key to the reproductive success of flowering plants. Meiosis and pollen formation are particularly sensitive to changes in growing conditions, especially temperature. We have previously identified cyclin-dependent kinase G1 (CDKG1) as a master regulator of temperature-dependent meiosis and this may involve the regulation of alternative splicing (AS), including of its own transcript. CDKG1 mRNA can undergo several AS events, potentially producing two protein variants: CDKG1L and CDKG1S, differing in their N-terminal domain which may be involved in co-factor interaction. In leaves, both isoforms have distinct temperature-dependent functions on target mRNA processing, but their role in pollen development is unknown. In the present study, we characterize the role of CDKG1L and CDKG1S in maintaining Arabidopsis fertility. We show that the long (L) form is necessary and sufficient to rescue the fertility defects of the cdkg1-1 mutant, while the short (S) form is unable to rescue fertility. On the other hand, an extra copy of CDKG1L reduces fertility. In addition, mutation of the ATP binding pocket of the kinase indicates that kinase activity is necessary for the function of CDKG1. Kinase mutants of CDKG1L and CDKG1S correctly localize to the cell nucleus and nucleus and cytoplasm, respectively, but are unable to rescue either the fertility or the splicing defects of the cdkg1-1 mutant. Furthermore, we show that there is partial functional overlap between CDKG1 and its paralog CDKG2 that could in part be explained by overlapping gene expression.
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