Breaking of plant stomatal one-cell-spacing rule by sugar solution immersion.
Breaking of plant stomatal one-cell-spacing rule by sugar solution immersion.
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DOI：
10.1371/journal.pone.0072456
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Higaki T
中科院分区：
文献类型：
--
作者：
Akita K;Hasezawa S;Higaki T
植物气孔的空间分布是研究表皮细胞格局形成的模型系统。分子遗传学方法已经确定了气孔分布模式所需的几个关键基因，但环境条件下，扰动气孔间距分布尚未确定。我们发现，在1 - 5%的蔗糖溶液中浸泡水培培养诱导拟南芥幼苗子叶中的气孔异常聚集。葡萄糖或果糖溶液处理也能诱导气孔紊乱，而甘露醇溶液处理则不能诱导气孔紊乱，说明渗透胁迫不是气孔紊乱的原因。气孔谱系细胞特异性增强子捕获系表明，糖溶液处理导致气孔谱系细胞特异性基因在非气孔谱系细胞中异位表达。苯胺蓝染色还表明，在气孔前体细胞（分生组织）形成过程中，新细胞壁中胼胝质（一种植物细胞壁成分）的沉积减少。这些结果表明，浸泡处理与糖溶液允许异位保卫细胞分化，通过功能障碍的细胞壁划分气孔和非气孔谱系细胞。本研究建立的简单的丛生气孔诱导系统为进一步研究植物气孔发育过程中的单细胞间距规律提供了一个合适的工具。
The spatial distribution of plant stomata is a model system to study epidermal cell pattern formation. Molecular genetic approaches have identified several key genes required for stomatal distribution patterning, but environmental conditions that perturb the stomatal spacing distribution have not yet been identified. We found that immersing hydroponic cultures in 1–5% sucrose solution induced abnormally clustered stomata in the cotyledons of Arabidopsis seedlings. Clustered stomata were also induced by treatment with glucose or fructose solution but not by mannitol solution, suggesting that osmotic stress was not a cause of the disturbed stomatal patterns. Stomatal lineage cell-specific enhancer trap lines revealed that the sugar solution treatment led to ectopic expression of stomatal lineage cell-specific genes in non-stomatal lineage cells. Aniline blue staining also showed that there was reduced deposition of callose, a plant cell wall component, in new cell walls during formation of stomatal precursor cells (meristemoids). These results suggested that the immersion treatment with sugar solution permitted ectopic guard cell differentiation through dysfunction of the cell wall dividing stomatal- and non-stomatal lineage cells. Our simple induction system for clustered stomata provides a suitable tool for further studies to investigate the one-cell-spacing rule during plant stomatal development.
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