Over-expression of microRNA171 affects phase transitions and floral meristem determinancy in barley.
Over-expression of microRNA171 affects phase transitions and floral meristem determinancy in barley.
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DOI：
10.1186/1471-2229-13-6
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发表时间：
2013-01-07
影响因子：
5.3
通讯作者：
Helliwell C
中科院分区：
文献类型：
--
作者：
Curaba J;Talbot M;Li Z;Helliwell C
从幼体到成体和成体到生殖生长阶段的过渡是植物生命周期中的重要阶段。这些转变的调控因子包括mirna，特别是miR156和miR172，它们是被子植物中保守的调控模块的一部分。在拟南芥中，miR171通过抑制SCL转录因子的表达来抑制腋下分生组织的分化，但在其他植物中尚未发现miR171的作用。为了研究mir171及其靶基因在单子叶中的作用，研究了Hvu pri-miR171a在大麦（Hordeum vulgare L. cv.）中的过表达。黄金承诺)导致至少一个HvSCL基因的表达减少。所获得的转基因植株表现出多效性表型，包括分枝缺陷、短营养植株数量增加和开花晚。这些表型似乎是茎分生组织变化的结果。此外，数据显示，miR171过表达通过激活miR156通路和抑制TRD （THIRD OUTER GLUME）和HvPLA1 （Plastochron1）基因的表达，改变了营养期到生殖期的转变。我们的数据表明，在拟南芥中确定的miR171及其靶基因的一些作用在大麦中是保守的，并且它们在大麦中具有额外的功能，包括激活miR156途径。
The transitions from juvenile to adult and adult to reproductive phases of growth are important stages in the life cycle of plants. The regulators of these transitions include miRNAs, in particular miR156 and miR172 which are part of a regulatory module conserved across the angiosperms. In Arabidopsis miR171 represses differentiation of axillary meristems by repressing expression of SCARECROW-LIKE(SCL) transcription factors, however the role of miR171 has not been examined in other plants. To investigate the roles of mir171 and its target genes in a monocot, the Hvu pri-miR171a was over-expressed in barley (Hordeum vulgare L. cv. Golden promise) leading to reduced expression of at least one HvSCL gene. The resulting transgenic plants displayed a pleiotropic phenotype which included branching defects, an increased number of short vegetative phytomers and late flowering. These phenotypes appear to be the consequence of changes in the organisation of the shoot meristem. In addition, the data show that miR171 over-expression alters the vegetative to reproductive phase transition by activating the miR156 pathway and repressing the expression of the TRD (THIRD OUTER GLUME) and HvPLA1 (Plastochron1) genes. Our data suggest that some of the roles of miR171 and its target genes that have been determined in Arabidopsis are conserved in barley and that they have additional functions in barley including activation of the miR156 pathway.
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影响因子：
7.4
作者：
Engstrom, Eric M.;Andersen, Carl M.;Bowman, John L.
通讯作者：
Bowman, John L.
影响因子：
7.2
作者：
Lee, Dong-Yeon;An, Gynheung
通讯作者：
An, Gynheung
影响因子：
7.2
作者：
Lee, Dong-Yeon;Lee, Jinwon;An, Gynheung
通讯作者：
An, Gynheung
DOI：
10.1073/pnas.0909097107
发表时间：
2010-01-05
影响因子：
11.1
作者：
Nair, Sudha K.;Wang, Ning;Komatsuda, Takao
通讯作者：
Komatsuda, Takao
影响因子：
5.1
作者：
Jung, Jae-Hoon;Seo, Pil Joon;Park, Chung-Mo
通讯作者：
Park, Chung-Mo