Characteristics of a human epidermal squamous carcinoma cell line at different extracellular calcium concentrations.
Characteristics of a human epidermal squamous carcinoma cell line at different extracellular calcium concentrations.
复制标题
不同细胞外钙浓度下人表皮鳞状癌细胞系的特征。
DOI：
10.1016/0014-4827(84)90576-7
复制
发表时间：
1984
影响因子：
3.7
通讯作者：
G. Walker
中科院分区：
文献类型：
--
作者：
M. Brysk;J. Miller;G. Walker
人皮肤鳞状细胞癌细胞系已在高、中、低三种钙离子浓度的介质中生长。细胞的生长速度不受钙离子水平的影响，但细胞形态发生了变化。低钙时桥粒缺失，高钙时可见细胞堆积。在三种钙离子水平下生长的细胞在聚丙烯酰胺凝胶上的蛋白质染色图谱是相同的。与~(125)I-ConA反应的糖蛋白变异较小。乳酸氧化物酶碘化显示细胞表面蛋白质的变化，最明显的是在高钙时出现新的蛋白质。
A continuous line derived from a human skin squamous cell carcinoma has been grown in media of high, normal and low Ca2+concentrations. The growth rate was unaffected by the Ca2+levels even though morphological changes were observed. Desmosomes were absent at low Ca2+and areas of cell piling were observed at high Ca2+. Cell protein staining patterns on polyacrylamide gels were identical for cells grown at the three Ca2+levels. The variations were minor for the glycoproteins reacted with125I-conA. Lactoper-oxidase iodination revealed changes in cell surface proteins, most markedly in the emergence of new proteins at high Ca2+.
影响因子：
11.2
作者：
J. Rheinwald;M. Beckett
通讯作者：
J. Rheinwald;M. Beckett
肿瘤促进剂和细胞外钙对正常、转化和温度敏感的大鼠肝上皮细胞生长的影响。
影响因子：
9.7
作者：
Fisher,PB;Weinstein,IB
通讯作者：
Weinstein,IB