The Long Non-Coding RNA ENST00000489676 Influences Papillary Thyroid Cancer Cell Proliferation and Invasion through Regulating MiR-922
The Long Non-Coding RNA ENST00000489676 Influences Papillary Thyroid Cancer Cell Proliferation and Invasion through Regulating MiR-922
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长非编码RNA ENST00000489676通过调节miR-922影响甲状腺乳头状癌细胞的增殖和侵袭
DOI：
10.7150/jca.31751
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发表时间：
2019-08
期刊：
影响因子：
--
通讯作者：
Weiwei Zhan
中科院分区：
文献类型：
--
作者：
Wenhan Wang;Shujun Xia;Weiwei Zhan
Purpose: Long noncoding RNAs (lncRNAs) have been reported to be associated with the growth and progression of numerous cancers. The aim of this study was to explore the potential function and molecular mechanism of lncRNA ENST00000489676 in the proliferation and invasion of papillary thyroid cancer (PTC). Methods: QRT-PCR (quantitative reverse transcriptase-polymerase chain reaction) was used to determine the levels of ENST00000489676. CCK-8 (Cell Counting Kit-8) assay and colony formation assay were performed to detect the cell proliferation. Flow cytometry was used to analyze the cell cycle. Transwell and scratch assay were performed to detect the migration and invasion ability. Results: The expression of ENST00000489676 was significantly overexpressed in TPC1 compared to KTC1 cell lines. When ENST00000489676 expression was knocked down, the proliferation, migration and invasion ability as well as cell cycle were all promoted in PTC cell lines, while those abilities were all suppressed when ENST00000489676 overexpressed. Overexpression of ENST00000489676 could inhibit cell proliferation, migration and invasion in vitro. Moreover, ENST00000489676 may mediate tumor suppression in PTC cells through suppressing miR-922. Conclusions: ENST00000489676 negatively regulated the proliferation, migration invasion and cell cycle of PTC. The overexpression of ENST00000489676 inhibited the progression of PTC through suppressing miR-922. ENST00000489676 could be act as a novel diagnosis marker and a potential therapeutic target for PTC.
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影响因子：
4.6
作者：
Ishibashi Y;Ohtsu H;Ikemura M;Kikuchi Y;Niwa T;Nishioka K;Uchida Y;Miura H;Aikou S;Gunji T;Matsuhashi N;Ohmoto Y;Sasaki T;Seto Y;Ogawa T;Tada K;Nomura S
通讯作者：
Nomura S
影响因子：
64.5
作者：
Cancer Genome Atlas Research Network
通讯作者：
Cancer Genome Atlas Research Network
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.12659/msm.908652
发表时间：
2018-07-03
期刊：
Medical science monitor : international medical journal of experimental and clinical research
影响因子：
--
作者：
Min X;Liu K;Zhu H;Zhang J
通讯作者：
Zhang J
DOI：
10.3233/cbm-181198
发表时间：
2018-10
期刊：
Cancer biomarkers : section A of Disease markers
影响因子：
--
作者：
Tao Zhou;Meijun Zhong;Shuyong Zhang;Zhi Wang;Rong Xie;Chengfeng Xiong;Y. Lv;Wan-zhi Chen;Jichun Yu
通讯作者：
Tao Zhou;Meijun Zhong;Shuyong Zhang;Zhi Wang;Rong Xie;Chengfeng Xiong;Y. Lv;Wan-zhi Chen;Jichun Yu