High-Level Heat Resistance of Spores of Bacillus amyloliquefaciens and Bacillus licheniformis Results from the Presence of a spoVA Operon in a Tn1546 Transposon.
High-Level Heat Resistance of Spores of Bacillus amyloliquefaciens and Bacillus licheniformis Results from the Presence of a spoVA Operon in a Tn1546 Transposon.
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DOI：
10.3389/fmicb.2016.01912
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发表时间：
2016
影响因子：
5.2
通讯作者：
Wells-Bennik MH
中科院分区：
文献类型：
--
作者：
Berendsen EM;Koning RA;Boekhorst J;de Jong A;Kuipers OP;Wells-Bennik MH
细菌内生孢子形成者可以产生对许多食品加工条件（包括热）具有抗性的孢子。一些孢子可以在旨在生产商业无菌食品的加热过程中存活。最近，显示存在于枯草芽孢杆菌中的Tn1546转座子上的spoVA操纵子（命名为spoVA2mob）导致B的耐湿热性显著增加。枯草芽孢在解淀粉芽孢杆菌和地衣芽孢杆菌的几种菌株中也发现了包括spoVA2mob操纵子的这种Tn1546转座子元件，并且这些菌株显示出比缺乏这种转座子的对应物产生对湿热具有显著更高抗性的孢子。在这项研究中，Tn1546转座子包围spoVA 2 mob操纵子在B中的位置和组成。解淀粉菌和B. B.分析了地衣型。将这些spoVA 2 mob操纵子引入B。枯草芽孢杆菌168（产生不具有高度耐热性的孢子）使突变体168菌株产生高水平耐热性孢子，证明这些元件在B.解淀粉菌和B. B.地衣菌负责孢子的高水平耐热性。在5.2°C和57.7°C之间对每个物种的9个菌株的生长进行评估，显示菌株之间存在一些差异，特别是在较低温度下，但所有菌株都能够在57.7°C下生长。B.解淀粉菌和B. B.含有Tn1546元件（并产生高水平耐热孢子）的地衣菌在与产生低水平耐热孢子的Tn1546阴性对应物相似的温度下生长。本研究中的发现允许检测B。解淀粉菌和B. B.地衣菌在食物链中产生高度耐热的孢子。
Bacterial endospore formers can produce spores that are resistant to many food processing conditions, including heat. Some spores may survive heating processes aimed at production of commercially sterile foods. Recently, it was shown that a spoVA operon, designated spoVA2mob, present on a Tn1546 transposon in Bacillus subtilis, leads to profoundly increased wet heat resistance of B. subtilis spores. Such Tn1546 transposon elements including the spoVA2mob operon were also found in several strains of Bacillus amyloliquefaciens and Bacillus licheniformis, and these strains were shown to produce spores with significantly higher resistances to wet heat than their counterparts lacking this transposon. In this study, the locations and compositions of Tn1546 transposons encompassing the spoVA2mob operons in B. amyloliquefaciens and B. licheniformis were analyzed. Introduction of these spoVA2mob operons into B. subtilis 168 (producing spores that are not highly heat resistant) rendered mutant 168 strains that produced high-level heat resistant spores, demonstrating that these elements in B. amyloliquefaciens and B. licheniformis are responsible for high level heat resistance of spores. Assessment of growth of the nine strains of each species between 5.2°C and 57.7°C showed some differences between strains, especially at lower temperatures, but all strains were able to grow at 57.7°C. Strains of B. amyloliquefaciens and B. licheniformis that contain the Tn1546 elements (and produce high-level heat resistant spores) grew at temperatures similar to those of their Tn1546-negative counterparts that produce low-level heat resistant spores. The findings presented in this study allow for detection of B. amyloliquefaciens and B. licheniformis strains that produce highly heat resistant spores in the food chain.
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影响因子：
5.2
作者：
Gopal N;Hill C;Ross PR;Beresford TP;Fenelon MA;Cotter PD
通讯作者：
Cotter PD
影响因子：
5.3
作者：
Berendsen, Erwin M.;Zwietering, Marcel H.;Wells-Bennik, Marjon H. J.
通讯作者：
Wells-Bennik, Marjon H. J.
影响因子：
4
作者：
Cazemier, AE;Wagenaars, SFM;ter Steeg, PF
通讯作者：
ter Steeg, PF
影响因子：
3.2
作者：
ARTHUR, M;MOLINAS, C;COURVALIN, P
通讯作者：
COURVALIN, P
影响因子：
11
作者：
Berendsen, Erwin M.;Boekhorst, Jos;Wells-Bennik, Marjon H. J.
通讯作者：
Wells-Bennik, Marjon H. J.