Mechanisms of salt tolerance in habanero pepper plants (Capsicum chinense Jacq.): Proline accumulation, ions dynamics and sodium root-shoot partition and compartmentation.
Mechanisms of salt tolerance in habanero pepper plants (Capsicum chinense Jacq.): Proline accumulation, ions dynamics and sodium root-shoot partition and compartmentation.
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DOI：
10.3389/fpls.2014.00605
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发表时间：
2014
影响因子：
5.6
通讯作者：
Martínez-Estévez M
中科院分区：
文献类型：
--
作者：
Bojórquez-Quintal E;Velarde-Buendía A;Ku-González A;Carillo-Pech M;Ortega-Camacho D;Echevarría-Machado I;Pottosin I;Martínez-Estévez M
尽管其经济意义，很少有人知道辣椒植物耐盐机制。为了解决这个问题，我们比较了两个辣椒品种：雷克斯（耐受）和奇琴-伊察（敏感）对NaCl的反应差异。在盐胁迫下（150 mM NaCl超过7天），雷克斯品种的根积累了比奇琴-伊察品种多50倍的相容性溶质，如脯氨酸。矿物分析表明，Na+被限制在根部，阻止其运输到叶片。荧光分析表明，一个有效的Na+区室化的液泡状结构和细胞内小隔间在根的雷克斯品种。同时，Na+在奇琴-伊察植物中被区室化在质外体中，表明大量的Na+挤出。通过对雷克斯（Rex）品种根系的矿物分析和微电极离子通量测定（MIFE），发现在盐胁迫下，雷克斯品种根系中的K+含量较高。钒酸盐敏感的H+流出量在奇琴-伊察品种植物中较高，表明质膜H+-ATP酶的活性较高，这促进了Na+的排出，并且可能也促进了K+的再吸收。我们的研究结果表明，胁迫耐受机制的组合，以减轻盐诱导的伤害。此外，Na+挤压到质外体似乎不是辣椒植物耐盐性的有效策略。
Despite its economic relevance, little is known about salt tolerance mechanisms in pepper plants. To address this question, we compared differences in responses to NaCl in two Capsicum chinense varieties: Rex (tolerant) and Chichen-Itza (sensitive). Under salt stress (150 mM NaCl over 7 days) roots of Rex variety accumulated 50 times more compatible solutes such as proline compared to Chichen-Itza. Mineral analysis indicated that Na+ is restricted to roots by preventing its transport to leaves. Fluorescence analysis suggested an efficient Na+ compartmentalization in vacuole-like structures and in small intracellular compartments in roots of Rex variety. At the same time, Na+ in Chichen-Itza plants was compartmentalized in the apoplast, suggesting substantial Na+ extrusion. Rex variety was found to retain more K+ in its roots under salt stress according to a mineral analysis and microelectrode ion flux estimation (MIFE). Vanadate-sensitive H+ efflux was higher in Chichen-Itza variety plants, suggesting a higher activity of the plasma membrane H+-ATPase, which fuels the extrusion of Na+, and, possibly, also the re-uptake of K+. Our results suggest a combination of stress tolerance mechanisms, in order to alleviate the salt-induced injury. Furthermore, Na+ extrusion to apoplast does not appear to be an efficient strategy for salt tolerance in pepper plants.
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影响因子：
4.3
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