Anti-Nogo-A antibody treatment promotes recovery of manual dexterity after unilateral cervical lesion in adult primates--re-examination and extension of behavioral data.
Anti-Nogo-A antibody treatment promotes recovery of manual dexterity after unilateral cervical lesion in adult primates--re-examination and extension of behavioral data.
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抗Nogo-A抗体治疗促进成年灵长类单侧颈椎损伤后手灵巧度的恢复——行为数据的重新检查和扩展。
DOI：
10.1111/j.1460-9568.2009.06642.x
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发表时间：
2009-03
期刊：
影响因子：
--
通讯作者：
Rouiller EM
中科院分区：
文献类型：
--
作者：
Freund P;Schmidlin E;Wannier T;Bloch J;Mir A;Schwab ME;Rouiller EM
在遭受脊髓损伤的啮齿动物和非人灵长类动物中，中和轴突生长抑制剂Nogo-A已被证明可促进再生轴突发芽和功能恢复。本报告的目的是重新检查数据的灵长类动物的手灵巧性的恢复使用精细的行为分析和进一步的统计评估，代表次要的结果措施，从相同的手灵巧性测试。研究人员对13只成年猴子进行了研究，其中7只接受了抗Nogo-A抗体，而其他猴子则接受了对照抗体。训练猴子执行改良的Brinkman板任务，该任务需要食指和拇指相对以抓取放置在垂直和水平定向槽中的食物颗粒。两个参数进行了量化之前和之后的脊髓损伤：（i）标准的“分数”，定义为从两种类型的插槽在30秒内检索的颗粒的数量;（ii）新引入的“接触时间”，定义为成功检索前的手指与食物颗粒接触的持续时间。病变后，所有猴子的手都严重受损，随后进行性功能恢复。值得注意的是，抗Nogo-A抗体处理的猴比对照抗体处理的猴恢复得更快且显著更好，考虑到垂直和水平槽的评分（Mann-Whitney检验：分别为P = 0.05和0.035）和接触时间（分别为P = 0.008和0.005）。病变的详细分析排除了这一结论可能是由两组猴之间病变性质差异引起的可能性。
In rodents and nonhuman primates subjected to spinal cord lesion, neutralizing the neurite growth inhibitor Nogo-A has been shown to promote regenerative axonal sprouting and functional recovery. The goal of the present report was to re-examine the data on the recovery of the primate manual dexterity using refined behavioral analyses and further statistical assessments, representing secondary outcome measures from the same manual dexterity test. Thirteen adult monkeys were studied; seven received an anti-Nogo-A antibody whereas a control antibody was infused into the other monkeys. Monkeys were trained to perform the modified Brinkman board task requiring opposition of index finger and thumb to grasp food pellets placed in vertically and horizontally oriented slots. Two parameters were quantified before and following spinal cord injury: (i) the standard ‘score’ as defined by the number of pellets retrieved within 30 s from the two types of slots; (ii) the newly introduced ‘contact time’ as defined by the duration of digit contact with the food pellet before successful retrieval. After lesion the hand was severely impaired in all monkeys; this was followed by progressive functional recovery. Remarkably, anti-Nogo-A antibody-treated monkeys recovered faster and significantly better than control antibody-treated monkeys, considering both the score for vertical and horizontal slots (Mann–Whitney test: P = 0.05 and 0.035, respectively) and the contact time (P = 0.008 and 0.005, respectively). Detailed analysis of the lesions excluded the possibility that this conclusion may have been caused by differences in lesion properties between the two groups of monkeys.
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影响因子：
5.7
作者：
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通讯作者：
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影响因子：
56.9
作者：
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2.5
作者：
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作者：
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影响因子：
2.4
作者：
Beaud, Marie-Laure;Schmidlin, Eric;Wannier, Thierry;Freund, Patrick;Bloch, Jocelyne;Mir, Anis;Schwab, Martin E.;Rouiller, Eric M.
通讯作者：
Rouiller, Eric M.