Analysis of the contribution of cellular and viral RNA to the packaging of APOBEC3G into HIV-1 virions.
Analysis of the contribution of cellular and viral RNA to the packaging of APOBEC3G into HIV-1 virions.
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DOI：
10.1186/1742-4690-4-48
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发表时间：
2007-07-16
期刊：
影响因子：
3.3
通讯作者：
Strebel K
中科院分区：
文献类型：
--
作者：
Khan MA;Goila-Gaur R;Opi S;Miyagi E;Takeuchi H;Kao S;Strebel K
细胞胞苷脱氨酶APOBEC 3G（APO 3G）有效掺入HIV-1病毒粒子是其抗病毒活性所必需的。尽管已知细胞RNA非特异性地掺入病毒颗粒中，但我们先前发现，病毒基因组RNA特异性地增强APO 3G掺入HIV-1病毒颗粒中。在细胞内，发现APO 3G形成涉及多种细胞RNA的大RNA-蛋白质复合物。本研究的目的是调查最近在细胞内APO 3G核糖核蛋白复合物中鉴定的宿主RNA对APO 3G向HIV-1病毒体的转化的可能贡献。我们的研究结果表明，7SL RNA，信号识别颗粒的组分，和hY 1，hY 3，hY 4，hY 5 RNA存在于细胞内APO 3G复合物中，并包装到缺乏病毒基因组RNA的HIV-1颗粒中，这与APO 3G不同，APO 3G没有大量包装到基因组RNA缺陷颗粒中。这些结果表明，7SL或hY RNA的包装不足以将APO 3G包装到HIV-1病毒体中。我们还测试了其他几种细胞RNA的折叠，包括β-肌动蛋白、GAPDH、α-微管蛋白和小核RNA，并确定了它们对APO 3G包装成新生病毒体的影响。同样，我们无法观察到APO 3G的糖苷化和这些细胞RNA的包装之间的任何相关性。本研究的结果支持我们先前的结论，即病毒基因组RNA是APO 3G掺入HIV-1病毒粒子的关键决定因素。虽然在这项研究中测试的大多数细胞RNA被包装到病毒或病毒样颗粒中，但我们未能确定APO 3G糖苷化与这些细胞RNA包装之间的相关性。
Efficient incorporation of the cellular cytidine deaminase APOBEC3G (APO3G) into HIV-1 virions is necessary for its antiviral activity. Even though cellular RNAs are known to be non-specifically incorporated into virus particles, we have previously found that encapsidation of APO3G into HIV-1 virions is specifically enhanced by viral genomic RNA. Intracellularly, APO3G was found to form large RNA-protein complexes involving a variety of cellular RNAs. The goal of this study was to investigate the possible contribution of host RNAs recently identified in intracellular APO3G ribonucleoprotein complexes to APO3G's encapsidation into HIV-1 virions. Our results show that 7SL RNA, a component of signal recognition particles, and hY1, hY3, hY4, hY5 RNAs were present in intracellular APO3G complexes and were packaged into HIV-1 particles lacking viral genomic RNA unlike APO3G, which was not packaged in significant amounts into genomic RNA-deficient particles. These results indicate that packaging of 7SL or hY RNAs is not sufficient for the packaging of APO3G into HIV-1 virions. We also tested the encapsidation of several other cellular RNAs including β-actin, GAPDH, α-tubulin, and small nuclear RNAs and determined their effect on the packaging of APO3G into nascent virions. Again, we were unable to observe any correlation between APO3G encapsidation and the packaging of any of these cellular RNAs. The results from this study support our previous conclusion that viral genomic RNA is a critical determinant for APO3G incorporation into HIV-1 virions. While most cellular RNAs tested in this study were packaged into viruses or virus-like particles we failed to identify a correlation between APO3G encapsidation and the packaging of these cellular RNAs.
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影响因子：
5.4
作者：
Karczewski, MK;Strebel, K
通讯作者：
Strebel, K
影响因子：
5.4
作者：
Kao, S;Khan, MA;Strebel, M
通讯作者：
Strebel, M
影响因子：
5.4
作者：
Clever, JL;Parslow, TG
通讯作者：
Parslow, TG
DOI：
10.1016/j.bbrc.2004.07.005
发表时间：
2004-08-27
影响因子：
3.1
作者：
Douaisi, M;Dussart, S;Decroly, E
通讯作者：
Decroly, E
影响因子：
3.7
作者：
ERIKSON, RL
通讯作者：
ERIKSON, RL