Interleukin 1 polymorphisms, lifestyle factors, and Helicobacter pylori infection.
Interleukin 1 polymorphisms, lifestyle factors, and Helicobacter pylori infection.
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DOI：
10.1111/j.1349-7006.2001.tb01106.x
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发表时间：
2001-04
期刊：
影响因子：
--
通讯作者：
Tominaga S
中科院分区：
文献类型：
--
作者：
Hamajima N;Matsuo K;Saito T;Tajima K;Okuma K;Yamao K;Tominaga S
几位作者报道了幽门螺杆菌感染与生活方式因素之间的关系，但对与感染相关的宿主因素知之甚少。本研究旨在通过白细胞介素（TL - IA）、IL - 1B和IL - 1RN基因多态性检测HP的感染率，并探讨其与生活方式因素的相互作用。研究对象为241名参加HP根除计划的非癌症门诊患者。利用PCR（聚合酶链式反应）、PCR - RFLP（限制性片段长度多态性）和PCR - cttp（双对引物PCR）对IL - 1A‐889位点（T - C）、IL - 1B‐31位点（C - T； T等位基因形成TATA盒）和IL - 1B‐511位点（C - T）以及IL - 1RN内含子2位点（86 - bp VNTR（可变数目串联重复序列））的多态性进行基因分型。结果发现，IL - 1B在‐31和‐511位点的多态性几乎完全相关，但与高加索人相反；‐31C/‐511T和‐31T/‐511C等位基因在本研究对象中占主导地位。不同IL - 1B C - 31T基因型的人HP感染率存在显著差异；45.2%（19/42）选择C/C， 67.7%（90/133）选择C/T， 63.6%（42/66）选择T/T。与C/C基因型相关的年龄-性别调整优势比（OR）为T/C基因型2.32(95%可信区间1.10‐4.92)，T/T基因型2.46（1.06‐5.74）。吸烟状况显著改变T/T基因型的OR值；相互作用项=14.6（1.12‐190）。IL - 1A和IL - 1RN的多态性与感染率无关。结果表明，IL - 1B C - 31T的T等位基因与持续HP感染的易感性有关，并且这种易感性会因吸烟而改变。
Associations between Helicobacter pylori (HP) infection and lifestyle factors have been reported by several authors, but little is known about the host factors associated with the infection. This study aims to examine the infection rate of HP according to gene polymorphisms of interleukin (TL)‐IA, IL‐1B, and IL‐1RN, and to investigate the interactions with lifestyle factors. Subjects were 241 non‐cancer outpatients who had participated in a HP eradication program. Polymorphisms at ‐889 (T to C) of IL‐1A, at ‐31 (C to T; T allele makes a TATA box) and ‐511 (C to T) of IL‐1B, and at intron 2 (86‐bp VNTR (variable number of tandem repeats)) of IL‐1RN were genotyped by PCR (polymerase chain reaction), PCR‐RFLP (restriction fragment length polymorphism) and PCR‐CTPP (PCR with confronting two‐pair primers). It was found that IL‐1B polymorphisms at ‐31 and ‐511 were near‐completely linked, but in the opposite way to that in Caucasians; ‐31C/ ‐511T and ‐31T/‐511C alleles were dominant in the present subjects. The HP infection rate was substantially different among the genotypes of IL‐1B C‐31T; 45.2% (19/42) for the C/C, 67.7% (90/133) for the C/T, and 63.6% (42/66) for the T/T. The age‐sex adjusted odds ratio (OR) relative to the C/C genotype was 2.32 (95%CI (confidence interval), 1.10‐4.92) for the T/C genotype and 2.46 (1.06‐5.74) for the T/T genotype. The OR for the T/T genotype was significantly modified by smoking status; interaction term=14.6 (1.12‐190). The polymorphisms of IL‐1A and IL‐1RN were not associated with the infection rate. The results suggested that the T allele of IL‐1B C‐31T is associated with vulnerability to persistent HP infection, and that the vulnerability is modified by smoking.
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影响因子：
24.5
作者：
Beales, ILP;Calam, J
通讯作者：
Calam, J
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3.8
作者：
BERGHOLDT, R;KARLSEN, AE;POCIOT, F
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Cave, DR
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作者：
EVANS, DJ;EVANS, DG;KLEIN, PD
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