Phylogeography and demographic history of Chinese black-spotted frog populations (Pelophylax nigromaculata): evidence for independent refugia expansion and secondary contact.
Phylogeography and demographic history of Chinese black-spotted frog populations (Pelophylax nigromaculata): evidence for independent refugia expansion and secondary contact.
复制标题
DOI：
10.1186/1471-2148-8-21
复制
发表时间：
2008-01-24
影响因子：
3.4
通讯作者：
Zhou K
中科院分区：
文献类型：
--
作者：
Zhang H;Yan J;Zhang G;Zhou K
更新世冰川对许多脊椎动物密切相关物种内部和之间的系统地理格局产生了相当大的影响。与欧洲和北美相比，东亚地区，特别是中国地区脊椎动物的系统地理研究还很有限。黑斑蛙是东亚分布较广的一种蛙类。该物种在中国的广泛分布使其成为研究古气候对东亚脊椎动物影响的理想模式。我们以前对黑斑对虾的研究发现，东北中国种群与大陆其他地区的种群之间存在着显着的分化。在目前的研究中，我们的目标是了解谱系之间最深的分裂以及随后的谱系划分是否在时间上与更新世的起源一致，以及在洞察扩张模式的情况下，分支地理分布是否与这一模型类似地在空间上一致。利用来自28个地点的262个个体的1143个核苷酸的线粒体细胞色素b基因，从简约分析中确定了两个主要分支(A分支和B分支)，它们的序列差异约为7.72%。相应的谱系分化时间为0.92Mya，表明最有可能是更新世的分裂。A支进一步划分为A1和A2两个亚支，层序差异为1.22%。嵌套的分支系统地理和种群人口分析表明，这种蛙种目前的分布是上一次间冰期两个独立避难所范围扩大的结果。通过核苷酸序列测定、聚合酶链式反应-限制性片段长度多态性和单倍型间简单序列重复序列分析，发现中国东辽地区黑斑对虾单倍型谱系A和B共存。ISSR结果特别支持线粒体A和B分支之间的分化，并暗示了两个不同谱系之间的内向基因流。嵌套的分支系统地理和种群人口分析表明，黑斑对虾目前的分布是晚更新世末次间冰期两个独立避难所范围扩大的结果。一个避难所在中国东部和西南高原的较低海拔。另一个线粒体分支的分布与朝鲜半岛存在避难所的情况一致。ISSR标记检测到的基因流表明，中国东北东辽地区两个避难所的范围扩大，两个高度分化的谱系之间存在二次接触。
Pleistocene glaciations had considerable impact on phylogeographic patterns within and among closely related species of many vertebrates. Compared to Europe and North America, research on the phylogeography of vertebrates in East Asia, particularly in China, remains limited. The black-spotted frog (Pelophylax nigromaculata) is a widespread species in East Asia. The wide distribution of this species in China makes it an ideal model for the study of palaeoclimatic effects on vertebrates in East Asia. Our previous studies of P. nigromaculata revealed significant subdivisions between the northeast China populations and populations in other regions of the mainland. In the present study, we aim to see whether the deepest splits among lineages and perhaps subsequent genealogical divisions are temporally consistent with a Pleistocene origin and whether clade geographic distributions, with insight into expansion patterns, are similarly spatially consistent with this model. Using 1143 nucleotides of the mitochondrial cytochrome b gene from 262 individuals sampled from 28 localities, two main clades (clade A and clade B) differing by c. 7.72% sequence divergence were defined from parsimony analyses. The corresponding timing of lineage divergence, 0.92 Mya, indicates a most likely Pleistocene split. The A clade is further subdivided into two sub-clades, A1 and A2 with 1.22% sequence divergence. Nested clade phylogeographical and population demographic analyses suggested that the current distribution of this frog species was the result of range expansion from two independent refugia during the last interglacial period. We discovered a population within which haplotype lineages A and B of P. nigromaculata coexist in the Dongliao area of China by nucleotide sequences, PCR-RFLP and ISSR (inter simple sequence repeat) patterns. The ISSR result in particular supported divergence between the mitochondrial clades A and B and implied introgressive gene flow between the two divergent lineages. Nested clade phylogeographical and population demographic analyses indicate that the current distribution of P. nigromaculata is the result of range expansion from two independent refugia during the last interglacial period in late Pleistocene. One refugium was in east China and the lower elevations of south-western plateau. The distribution of the other mitochondrial clade is consistent with the presence of a refugium in the Korean Peninsula. The gene flow as detected by ISSR markers suggests a range expansion of the two refugia and a secondary contact between the two highly divergent lineages in the Dongliao (DL) area of northeast China.
登录
查看更多内容
影响因子：
4.9
作者：
Clement, M;Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
4.9
作者：
Leache, Adam D.;Cole, Charles J.
通讯作者：
Cole, Charles J.
影响因子：
4.1
作者：
Austin, JD;Lougheed, SC;Boag, PT
通讯作者：
Boag, PT
影响因子：
64.8
作者：
Bard, E;Hamelin, B;Rougerie, F
通讯作者：
Rougerie, F
DOI：
10.1098/rspb.1998.0317
发表时间：
1998-03-22
影响因子：
4.7
作者：
Avise, JC;Walker, D
通讯作者：
Walker, D