Spectral radiation dependent photoprotective mechanism in the diatom Pseudo-nitzschia multistriata.
Spectral radiation dependent photoprotective mechanism in the diatom Pseudo-nitzschia multistriata.
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DOI：
10.1371/journal.pone.0087015
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ruban AV
中科院分区：
文献类型：
--
作者：
Brunet C;Chandrasekaran R;Barra L;Giovagnetti V;Corato F;Ruban AV
浮游植物，如硅藻，在海洋水柱中经历着光子通量密度(PFD)和光谱的巨大变化。硅藻已经形成了一些快速调节的光保护机制，如叶黄素循环激活(XC)和非光化学叶绿素荧光猝灭(NPQ)，以保护自己免受过量PFD引起的光氧化损伤。在这项研究中，我们研究了蓝光注量率和红光辐射在形成沿海假多条纹硅藻的光适应和保护反应中的作用。该种硅藻适应四种光谱光条件(蓝、红、蓝红、蓝红绿)，每种条件都具有低和高的PFD。我们的结果表明，XC池大小和NPQ幅度的增加是由细胞经历的蓝色注量率决定的，而细胞需要感应红色辐射来允许这些过程的发展。光谱的变化和蓝光与红光辐射的变化，或者调节了硅藻的光保护能力，如激活了双黄素-双黄素叶黄素循环、双黄素脱环氧化速率和非光化学猝灭能力，或者调节了这种硅藻的色素组成。我们认为，光的光谱组成对硅藻精细平衡光捕获和光保护能力的能力起着关键作用。
Phytoplankton, such as diatoms, experience great variations of photon flux density (PFD) and light spectrum along the marine water column. Diatoms have developed some rapidly-regulated photoprotective mechanisms, such as the xanthophyll cycle activation (XC) and the non-photochemical chlorophyll fluorescence quenching (NPQ), to protect themselves from photooxidative damages caused by excess PFD. In this study, we investigate the role of blue fluence rate in combination with red radiation in shaping photoacclimative and protective responses in the coastal diatom Pseudo-nitzschia multistriata. This diatom was acclimated to four spectral light conditions (blue, red, blue-red, blue-red-green), each of them provided with low and high PFD. Our results reveal that the increase in the XC pool size and the amplitude of NPQ is determined by the blue fluence rate experienced by cells, while cells require sensing red radiation to allow the development of these processes. Variations in the light spectrum and in the blue versus red radiation modulate either the photoprotective capacity, such as the activation of the diadinoxanthin-diatoxanthin xanthophyll cycle, the diadinoxanthin de-epoxidation rate and the capacity of non-photochemical quenching, or the pigment composition of this diatom. We propose that spectral composition of light has a key role on the ability of diatoms to finely balance light harvesting and photoprotective capacity.
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影响因子：
3.7
作者：
Schellenberger Costa B;Sachse M;Jungandreas A;Bartulos CR;Gruber A;Jakob T;Kroth PG;Wilhelm C
通讯作者：
Wilhelm C
影响因子：
2.1
作者：
Fujiki, T;Taguchi, S
通讯作者：
Taguchi, S
影响因子：
3.1
作者：
EILERS, PHC;PEETERS, JCH
通讯作者：
PEETERS, JCH
影响因子：
4.3
作者：
Gundermann, Kathi;Schmidt, Matthias;Buechel, Claudia
通讯作者：
Buechel, Claudia
影响因子：
9.4
作者：
Halsey, Kimberly H.;O'Malley, Robert T.;Behrenfeld, Michael J.
通讯作者：
Behrenfeld, Michael J.