Aureochrome 1a is involved in the photoacclimation of the diatom Phaeodactylum tricornutum.
Aureochrome 1a is involved in the photoacclimation of the diatom Phaeodactylum tricornutum.
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DOI：
10.1371/journal.pone.0074451
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wilhelm C
中科院分区：
文献类型：
--
作者：
Schellenberger Costa B;Sachse M;Jungandreas A;Bartulos CR;Gruber A;Jakob T;Kroth PG;Wilhelm C
金色素构成蓝光（BL）受体的家族，其仅在异鞭毛藻如硅藻（硅藻纲）和黄绿藻（黄藻纲）中发现。前人对三角褐指藻（Phaeodactylum tricornutum）的研究表明，其强光适应表型的形成是由BL的吸收介导的，而金色素可能在这一过程中起重要作用。三角褐指藻有四个编码金色素的基因。在这项研究中，我们证实了PtAUREO 1a，1b和2蛋白的核定位。此外，我们研究了转基因三角褐指藻细胞系的光质驯化生理与减少金色素1a基因的表达。结果表明，AUREO1a蛋白在光适应中具有独特的功能。然而，出乎意料的是，Aureo1a似乎抑制强光适应，这导致了Aureo1a沉默菌株的“超”强光适应状态。这是由几个光合参数的特征变化，包括增加最大光合速率，减少叶绿素a含量每细胞和增加值的非光化学猝灭AUREO1a沉默菌株相比，野生型文化。引人注目的是，在BL和红光（RL）条件下培养期间，与野生型细胞相比，AUREO1a沉默菌株表现出表型差异。因此，AUREO1a可能会影响RL信号传导过程，表明AUREO1a与RL感知通路的相互作用。
Aureochromes constitute a family of blue light (BL) receptors which are found exclusively in heterokont algae such as diatoms (Bacillariophyceae) and yellow-green algae (Xanthophyceae). Previous studies on the diatom Phaeodactylum tricornutum indicate that the formation of a high light acclimated phenotype is mediated by the absorption of BL and that aureochromes might play an important role in this process. P. tricornutum possesses four genes encoding aureochromes. In this study we confirm the nuclear localisation of the PtAUREO1a, 1b and 2 proteins. Furthermore we studied the physiology of light quality acclimation in genetically transformed P. tricornutum cell lines with reduced expression of the aureochrome 1a gene. The results demonstrate that the AUREO1a protein has a distinct function in light acclimation. However, rather unexpectedly AUREO1a seems to repress high light acclimation which resulted in a state of ‘hyper’ high light acclimation in aureo1a silenced strains. This was indicated by characteristic changes of several photosynthetic parameters, including increased maximum photosynthesis rates, decreased chlorophyll a contents per cell and increased values of non-photochemical quenching in AUREO1a silenced strains compared to wild type cultures. Strikingly, AUREO1a silenced strains exhibited phenotypic differences compared to wild type cells during cultivation under BL as well as under red light (RL) conditions. Therefore, AUREO1a might influence the RL signalling process, suggesting an interaction of AUREO1a with RL perception pathways.
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影响因子：
2.9
作者：
Braeutigam, Katharina;Dietzel, Lars;Pfannschmidt, Thomas
通讯作者：
Pfannschmidt, Thomas
影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
Rokhsar, DS
影响因子：
11.6
作者：
Braeutigam, Katharina;Dietzel, Lars;Pfannschmidt, Thomas
通讯作者：
Pfannschmidt, Thomas
影响因子：
64.8
作者：
Bowler, Chris;Allen, Andrew E.;Grigoriev, Igor V.
通讯作者：
Grigoriev, Igor V.
DOI：
10.1073/pnas.1300962110
发表时间：
2013-04-30
影响因子：
11.1
作者：
Ashworth, Justin;Coesel, Sacha;Baliga, Nitin S.
通讯作者：
Baliga, Nitin S.