Investigating the Mechanisms of Action of Depside Salt from Salvia miltiorrhiza Using Bioinformatic Analysis.
Investigating the Mechanisms of Action of Depside Salt from Salvia miltiorrhiza Using Bioinformatic Analysis.
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利用生物信息学分析研究丹参缩酚盐的作用机制
DOI：
10.1155/2017/5671860
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Li H;Liu H
丹参是一种用于治疗心血管疾病的传统中药。S.丹参（DSSM）含有以下活性成分：紫草酸镁B、紫草酸和迷迭香酸。本研究旨在揭示DSSM的作用机制。在GeneCards、Search Tool for Interacting Chemicals、SuperTarget、PubChem和Comparative Toxicogenomics Database中搜索DSSM相关基因后，使用Multifaceted Analysis Tool for Human Transcriptome对其进行富集分析。蛋白质-蛋白质相互作用（PPI）网络可视化;使用Cytoscape软件进行模块分析。最后，利用TRRUST数据库和Celloscape构建了转录调控网络。共鉴定出73个DSSM相关基因。JUN、TNF、NFKB 1和FOS是PPI网络中的枢纽节点。通过路径富集分析，从PPI网络中鉴定了模块1和2，表明模块1中存在NF KB 1和BCL 2表明与NF-κB信号传导路径的特定关联。JUN、TNF、NFKB 1、FOS和BCL 2在PPI网络中表现出显著的相互作用。在调控网络中还发现了JUN → TNF/FOS、FOS → NFKB 1和NFKB 1 → BCL 2/TNF等多种调控关系。因此，DSSM通过靶向JUN、TNF、NFKB 1、FOS和BCL 2发挥抗心血管疾病的作用。
Salvia miltiorrhiza is a traditional Chinese medicinal herb used for treating cardiovascular diseases. Depside salt from S. miltiorrhiza (DSSM) contains the following active components: magnesium lithospermate B, lithospermic acid, and rosmarinic acid. This study aimed to reveal the mechanisms of action of DSSM. After searching for DSSM-associated genes in GeneCards, Search Tool for Interacting Chemicals, SuperTarget, PubChem, and Comparative Toxicogenomics Database, they were subjected to enrichment analysis using Multifaceted Analysis Tool for Human Transcriptome. A protein-protein interaction (PPI) network was visualised; module analysis was conducted using the Cytoscape software. Finally, a transcriptional regulatory network was constructed using the TRRUST database and Cytoscape. Seventy-three DSSM-associated genes were identified. JUN, TNF, NFKB1, and FOS were hub nodes in the PPI network. Modules 1 and 2 were identified from the PPI network, with pathway enrichment analysis, showing that the presence of NFKB1 and BCL2 in module 1 was indicative of a particular association with the NF-κB signalling pathway. JUN, TNF, NFKB1, FOS, and BCL2 exhibited notable interactions among themselves in the PPI network. Several regulatory relationships (such as JUN → TNF/FOS, FOS → NFKB1 and NFKB1 → BCL2/TNF) were also found in the regulatory network. Thus, DSSM exerts effects against cardiovascular diseases by targeting JUN, TNF, NFKB1, FOS, and BCL2.
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影响因子：
4.6
作者：
Han H;Shim H;Shin D;Shim JE;Ko Y;Shin J;Kim H;Cho A;Kim E;Lee T;Kim H;Kim K;Yang S;Bae D;Yun A;Kim S;Kim CY;Cho HJ;Kang B;Shin S;Lee I
通讯作者：
Lee I
DOI：
10.1016/j.amjmed.2013.02.016
发表时间：
2013-08
期刊：
The American journal of medicine
影响因子：
--
作者：
Solomon DH;Curtis JR;Saag KG;Lii J;Chen L;Harrold LR;Herrinton LJ;Graham DJ;Kowal MK;Kuriya B;Liu L;Griffin MR;Lewis JD;Rassen JA
通讯作者：
Rassen JA
影响因子：
14.9
作者：
Keshava Prasad TS;Goel R;Kandasamy K;Keerthikumar S;Kumar S;Mathivanan S;Telikicherla D;Raju R;Shafreen B;Venugopal A;Balakrishnan L;Marimuthu A;Banerjee S;Somanathan DS;Sebastian A;Rani S;Ray S;Harrys Kishore CJ;Kanth S;Ahmed M;Kashyap MK;Mohmood R;Ramachandra YL;Krishna V;Rahiman BA;Mohan S;Ranganathan P;Ramabadran S;Chaerkady R;Pandey A
通讯作者：
Pandey A
影响因子：
14.9
作者：
Kim S;Thiessen PA;Bolton EE;Chen J;Fu G;Gindulyte A;Han L;He J;He S;Shoemaker BA;Wang J;Yu B;Zhang J;Bryant SH
通讯作者：
Bryant SH
影响因子：
4.3
作者：
Palomer X;Capdevila-Busquets E;Botteri G;Davidson MM;Rodríguez C;Martínez-González J;Vidal F;Barroso E;Chan TO;Feldman AM;Vázquez-Carrera M
通讯作者：
Vázquez-Carrera M