Ecology drives the evolution of diverse social strategies in Pseudomonas aeruginosa.
Ecology drives the evolution of diverse social strategies in Pseudomonas aeruginosa.
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DOI：
10.1111/mec.16119
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发表时间：
2021-10
影响因子：
4.9
通讯作者：
Kummerli, Rolf
中科院分区：
文献类型：
--
作者：
Figueiredo, Alexandre R. T.;Wagner, Andreas;Kummerli, Rolf
细菌通常通过分泌分子进行合作，这些分子可以作为公共物品在细胞之间共享。由于公共产品的生产容易受到突变体的欺骗，这些突变体利用公共产品而不为公共产品做出贡献，因此人们对阐明维持合作的进化力量产生了极大的兴趣。然而，在作弊不太可能重要的条件下，细菌合作是如何演变的，人们对此知之甚少。在这里，我们使用实验进化来跟踪细菌铜绿假单胞菌的模型公共产品铁清除铁载体绿脓菌荧光素的生产变化。在9种不同的环境中进化了1200代之后，我们观察到作弊者只在铁可用性低的液体介质中达到高频率。相反，当添加铁以降低生产绿脓菌荧光素的成本时，我们观察到对绿脓菌荧光素超生产者的选择。类似地，超级生产者也在高粘性介质中进化的种群中传播，其中相互作用的个体之间的相关性增加。进化克隆的全基因组测序显示，超生产与涉及编码群体感应通讯系统的基因的突变有关，而作弊克隆在铁饥饿西格玛因子或绿脓菌荧光素生物合成基因中存在突变。我们的研究结果表明，细菌的社会特征可以在不同的方向上迅速进化，特别是在个体分散减少的环境中，对合作水平的提高有很强的选择，正如社会进化理论所预测的那样。此外，我们建立了绿脓菌荧光素生产和群体感应之间的调节联系，表明一种性状（绿脓菌荧光素）的合作增加可能与另一种社会性状的丧失（群体感应）有关。
Bacteria often cooperate by secreting molecules that can be shared as public goods between cells. Because the production of public goods is subject to cheating by mutants that exploit the good without contributing to it, there has been great interest in elucidating the evolutionary forces that maintain cooperation. However, little is known about how bacterial cooperation evolves under conditions where cheating is unlikely to be of importance. Here we use experimental evolution to follow changes in the production of a model public good, the iron‐scavenging siderophore pyoverdine, of the bacterium Pseudomonas aeruginosa. After 1200 generations of evolution in nine different environments, we observed that cheaters only reached high frequency in liquid medium with low iron availability. Conversely, when adding iron to reduce the cost of producing pyoverdine, we observed selection for pyoverdine hyperproducers. Similarly, hyperproducers also spread in populations evolved in highly viscous media, where relatedness between interacting individuals is increased. Whole‐genome sequencing of evolved clones revealed that hyperproduction is associated with mutations involving genes encoding quorum‐sensing communication systems, while cheater clones had mutations in the iron‐starvation sigma factor or in pyoverdine biosynthesis genes. Our findings demonstrate that bacterial social traits can evolve rapidly in divergent directions, with particularly strong selection for increased levels of cooperation occurring in environments where individual dispersal is reduced, as predicted by social evolution theory. Moreover, we establish a regulatory link between pyoverdine production and quorum‐sensing, showing that increased cooperation with respect to one trait (pyoverdine) can be associated with the loss (quorum‐sensing) of another social trait.
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影响因子：
14.9
作者：
Balasubramanian D;Schneper L;Kumari H;Mathee K
通讯作者：
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作者：
Brockhurst MA;Buckling A;Racey D;Gardner A
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作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
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4.8
作者：
Clark, JS
通讯作者：
Clark, JS