NADPH production in dark stages is critical for cyanobacterial photocurrent generation: a study using mutants deficient in oxidative pentose phosphate pathway
NADPH production in dark stages is critical for cyanobacterial photocurrent generation: a study using mutants deficient in oxidative pentose phosphate pathway
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黑暗阶段 NADPH 的产生对于蓝藻光电流的产生至关重要：一项使用氧化戊糖磷酸途径缺陷的突变体的研究
DOI：
10.1007/s11120-022-00903-0
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发表时间：
2022
影响因子：
3.7
通讯作者：
Shimakawa Ginga
中科院分区：
文献类型：
--
作者：
Hatano Jiro;Kusama Shoko;Tanaka Kenya;Kohara Ayaka;Miyake Chikahiro;Nakanishi Shuji;Shimakawa Ginga
集成到细胞外电极上的活蓝细菌和藻类可以产生光感应电流（即光电流）。尽管预计光电流与光合细胞的氧化还原环境相关，但人们对光电流与细胞氧化还原状态之间的关系知之甚少。在这里，我们使用氧化戊糖磷酸（OPP）途径（OPP）途径（OPP）途径（在黑暗中产生NADPH的代谢途径）缺陷的几种突变体（Δzwf、Δgnd和ΔglgP）研究了蓝藻细胞的还原烟酰胺腺嘌呤二核苷酸磷酸[NADP(H)]氧化还原水平（光照前）对光电流的影响。蓝藻集胞藻中的 NAD(P)H 氧化还原水平和光电流。 PCC 6803 采用无创测量。 OPP 途径的功能障碍导致光合 NADPH 库在黑暗中氧化。此外，与野生型细胞相比，Δzwf、Δgnd和ΔglgP中的光电流诱导被延迟并且电流密度更低。外源添加的葡萄糖补偿了ΔglgP的表型并驱动突变体中的OPP途径，导致光电流增加。结果表明，光照前OPP途径积累的NADPH是光电流产生的关键因素。此外，测量光电流可以作为一种非侵入性方法来估计蓝藻中与 NADP(H) 池相关的细胞氧化还原水平。
Live cyanobacteria and algae integrated onto an extracellular electrode can generate a light-induced current (i.e., a photocurrent). Although the photocurrent is expected to be correlated with the redox environment of the photosynthetic cells, the relationship between the photocurrent and the cellular redox state is poorly understood. Here, we investigated the effect of the reduced nicotinamide adenine dinucleotide phosphate [NADP(H)] redox level of cyanobacterial cells (before light exposure) on the photocurrent using several mutants (Δzwf, Δgnd, and ΔglgP) deficient in the oxidative pentose phosphate (OPP) pathway, which is the metabolic pathway that produces NADPH in darkness. The NAD(P)H redox level and photocurrent in the cyanobacteriumSynechocystissp. PCC 6803 were measured noninvasively. Dysfunction of the OPP pathway led to oxidation of the photosynthetic NADPH pool in darkness. In addition, photocurrent induction was retarded and the current density was lower in Δzwf, Δgnd, and ΔglgPthan in wild-type cells. Exogenously added glucose compensated the phenotype of ΔglgPand drove the OPP pathway in the mutant, resulting in an increase in the photocurrent. The results indicated that NADPH accumulated by the OPP pathway before illumination is a key factor for the generation of a photocurrent. In addition, measuring the photocurrent can be a non-invasive approach to estimate the cellular redox level related to NADP(H) pool in cyanobacteria.
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影响因子：
5.6
作者：
Shimakawa G;Kohara A;Miyake C
通讯作者：
Miyake C
蓝藻细长聚球藻PCC7942内源氧化还原节律的电化学研究
DOI：
--
发表时间：
2020
期刊：
影响因子：
--
作者：
田中謙也;嶋中西周次
通讯作者：
嶋中西周次
影响因子：
3.3
作者：
Bradley, Robert W.;Bombelli, Paolo;Howe, Christopher J.
通讯作者：
Howe, Christopher J.
影响因子：
3.7
作者：
Tanaka K;Shimakawa G;Tabata H;Kusama S;Miyake C;Nakanishi S
通讯作者：
Nakanishi S
DOI：
10.1016/0006-291x(72)90527-x
发表时间：
1972-01-01
影响因子：
3.1
作者：
GRIMME, LH;BOARDMAN, NK
通讯作者：
BOARDMAN, NK