Disease association with two Helicobacter pylori duplicate outer membrane protein genes, homB and homA.
Disease association with two Helicobacter pylori duplicate outer membrane protein genes, homB and homA.
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DOI：
10.1186/1757-4749-1-12
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发表时间：
2009-06-22
期刊：
影响因子：
4.2
通讯作者：
Ménard A
中科院分区：
文献类型：
--
作者：
Oleastro M;Cordeiro R;Yamaoka Y;Queiroz D;Mégraud F;Monteiro L;Ménard A
homB 编码幽门螺杆菌外膜蛋白。该基因先前被认为与消化性溃疡病 (PUD) 相关，并被证明可在体外诱导白细胞介素 8 分泌的激活，并有助于细菌粘附。其 90% 相似的基因 homA 先前被认为与胃炎相关。本研究旨在评估从东亚和西方国家患者中分离出的 415 株幽门螺杆菌菌株中与 homB 和 homA 以及幽门螺杆菌毒力因子 cagA、babA 和 vacA 之间的胃病关联。还评估了这些基因型之间的相关性。 homB 和 homA 基因在世界范围内分布不均匀，东亚和西方菌株之间存在显着差异。在西方菌株中（n = 234、124 PUD 和 110 非溃疡性消化不良 (NUD)），homB、cagA 和 vacA s1 均与 PUD 显着相关（分别为 p = 0.025、p = 0.014、p = 0.039），并且 homA 与 NUD 密切相关（p = 0.072）。在东亚菌株中（n = 138、73 PUD 和 65 NUD），homB 的发现频率高于 homA，并且这些基因均与临床结果无关。总体而言，homB 与 cagA (p = 0.043) 和 vacA s1 (p < 0.001) 的存在相关，而 homA 在 cagA 阴性 (p = 0.062) 和 vacA s2 (p < 0.001) 菌株中更常见。 homB 和 homA 拷贝数多态性 观察到，具有明显的地理特异性，表明这些基因参与宿主适应。还观察到 homB 双拷贝基因型与 PUD 之间的相关性，强调了 homB 在菌株毒力中的作用。总体结果表明 homB 和 homA 有助于临床结果的确定。
homB encodes a Helicobacter pylori outer membrane protein. This gene was previously associated with peptic ulcer disease (PUD) and was shown to induce activation of interleukin-8 secretion in vitro, as well as contributing to bacterial adherence. Its 90%-similar gene, homA, was previously correlated with gastritis. The present study aimed to evaluate the gastric disease association with homB and homA, as well as with the H. pylori virulence factors cagA, babA and vacA, in 415 H. pylori strains isolated from patients from East Asian and Western countries. The correlation among these genotypes was also evaluated. Both homB and homA genes were heterogeneously distributed worldwide, with a marked difference between East Asian and Western strains. In Western strains (n = 234, 124 PUD and 110 non-ulcer dyspepsia (NUD), homB, cagA and vacA s1 were all significantly associated with PUD (p = 0.025, p = 0.014, p = 0.039, respectively), and homA was closely correlated with NUD (p = 0.072). In East Asian strains (n = 138, 73 PUD and 65 NUD), homB was found more frequently than homA, and none of these genes was associated with the clinical outcome. Overall, homB was associated with the presence of cagA (p = 0.043) and vacA s1 (p < 0.001), whereas homA was found more frequently in cagA-negative (p = 0.062) and vacA s2 (p < 0.001) strains. Polymorphisms in homB and homA copy number were observed, with a clear geographical specificity, suggesting an involvement of these genes in host adaptation. A correlation between the homB two-copy genotype and PUD was also observed, emphasizing the role of homB in the virulence of the strain. The global results suggest that homB and homA contribute to the determination of clinical outcome.
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影响因子：
24.5
作者：
Maeda, S;Ogura, K;Omata, M
通讯作者：
Omata, M
DOI：
10.1073/pnas.0308573100
发表时间：
2004-02-17
影响因子：
11.1
作者：
Solnick, JV;Hansen, LM;Syvanen, M
通讯作者：
Syvanen, M
影响因子：
9.4
作者：
Oliveira, AG;Santos, A;Queiroz, DMM
通讯作者：
Queiroz, DMM
影响因子：
24.5
作者：
Stolte, M;Bayerdörffer, E;Neubauer, A
通讯作者：
Neubauer, A
DOI：
10.1073/pnas.96.22.12778
发表时间：
1999-10-26
影响因子：
11.1
作者：
Gerhard, M;Lehn, N;Prinz, C
通讯作者：
Prinz, C