Profilin, a weak CAP for actin and RAS
Profilin, a weak CAP for actin and RAS
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Profilin，肌动蛋白和 RAS 的弱 CAP
DOI：
10.1016/0092-8674(81)90002-7
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发表时间：
1991
期刊：
影响因子：
64.5
通讯作者：
P. Janmey
中科院分区：
文献类型：
--
作者：
P. Goldschmidt;P. Janmey
许多细胞，如血小板和成纤维细胞，在细胞外激动剂的作用下改变形状和运动。这被认为是至关重要的一步，因为它经常发生在细胞致力于完成功能、生长甚至转化的时候。关于参与细胞信号传导的膜事件，我们已经了解了很多（Cantley et al., 1991）。同时，关于控制细胞形状和运动的细胞骨架结构也有了重大发现（Stossel, 1989; Pollard, 1990）。膜活性和细胞骨架结构之间的联系仍然是一个谜。然而，肌动蛋白骨架蛋白与膜信号通路关键分子之间的特异性相互作用已经在体外和体内被确定：肌动蛋白结合蛋白谱可能参与酿酒酵母的信号通路，该信号通路受RAS和腺苷环化酶（CAP）调控，RAS是一种普遍存在的GTPase，在不同细胞的分化、生长和增殖中起着核心作用（Hall, 1990）。在酿酒酵母中，RAS是唯一被发现的RAS效应细胞，RAS激活腺苷酸环化酶。这种效应特别需要CAP的n端结构域（Gerst et al., 1991；图1）。无CAP或其c端结构域的酵母细胞的表型类似于因基因破坏而耗尽profilin的酵母细胞。它们生长缓慢，变得非常大而圆，并且有萌芽和营养缺陷。在酵母细胞中为零
Many cells such as platelets and fibroblasts change their shape and move in response to extracellular agonists. This is considered to be a crucial step, because it often occurs when cells become committed to the accomplishment of a function, growth, or even transformation. Much has been learned about the membrane events involved in cell signaling (Cantley et al., 1991). Concurrently, major discoveries have been made concerning the structure of the cytoskeleton that controls cell shape and motility (Stossel, 1989; Pollard, 1990). The connection between membrane activity and cytoskeletal structure has remained a mystery. However, specific interactions between proteins of the actin-based skeleton and key molecules of membrane signaling pathways have been identified in vitro and now in vivo: the actin-binding protein profilin may contribute to a signaling pathway in Saccharomyces cerevisiae that is regulated by RAS, the ubiquitous GTPase that plays a central role in differentiation, growth, and proliferation of diverse cells (Hall, 1990) and by the adenyl cyclase-associated protein known as CAP (Vojtek et al., 1991). In S. cerevisiae, the only cell in which an effector of RAS has been characterized, RAS activates adenylate cyclase. This effect specifically requires the N-terminal domain of CAP (Gerst et al., 1991; Figure 1). The phenotype of yeast cells that are null for CAP or its C-terminal domain resembles that of yeast cells depleted of profilin by gene disruption. They grow slowly, become very large and round, and have budding and nutritional defects. In yeast cells null for
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细胞质蛋白以磷脂依赖性方式抑制 H-Ras 的 GTP 酶活性。
DOI：
10.1126/science.2237442
发表时间：
1990
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Tsai,MH;Yu,CL;Stacey,DW
通讯作者：
Stacey,DW
影响因子：
56.9
作者：
GOLDSCHMIDTCLERMONT, PJ;MACHESKY, LM;POLLARD, TD
通讯作者：
POLLARD, TD
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Stossel,TP
通讯作者：
Stossel,TP
影响因子：
56.9
作者：
YU, FX;JOHNSTON, PA;YIN, HL
通讯作者：
YIN, HL