Reactivity of direct assays for low-density lipoprotein (LDL) cholesterol toward charge-modified LDL in hypercholesterolemia.
Reactivity of direct assays for low-density lipoprotein (LDL) cholesterol toward charge-modified LDL in hypercholesterolemia.
复制标题
低密度脂蛋白 (LDL) 胆固醇直接测定对高胆固醇血症中电荷修饰 LDL 的反应性。
DOI：
10.1253/circj.cj-12-0381
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
K. Saku
中科院分区：
文献类型：
--
作者：
Bo Zhang;Emi Kawachi;A. Matsunaga;S. Imaizumi;Keita Noda;Y. Uehara;S. Miura;K. Yoshinaga;M. Kuroki;K. Saku
背景
本研究的目的是比较2种直接测量血清低密度脂蛋白胆固醇（LDL-C）与弗里德瓦尔德计算（LDL-C [F]）的方法，以及它们与高胆固醇血症（HC）患者血清超离心组分中基于大小和电荷的LDL亚组分的关系。
方法和结果
分析了参加他汀类药物试验（PATROL试验）的283例HC患者的血清样本。使用Sekisui Medical（LDL-C [Se]）和Kyowa Medex（LDL-C [Ky]）的试剂进行LDL-C的均相测定。通过毛细管等速电泳（cITP）分析基于电荷的LDL亚组分。在基线时HC患者的全血清中，LDL-C（Se）和LDL-C（Ky）分别与LDL-C（F）负偏离和正偏离。LDL-C（Se）与LDL-C（F）的负偏差随LDL-C的升高而增加，而LDL-C（Ky）与LDL-C（F）的正偏差与cITP分析的电荷修饰LDL（cmLDL）呈正相关。在血清d>1.006 g/ml和>1.040 g/ml的组分（分别为LDL和小而密的LDL组分）中，LDL-C（Ky）与LDL-C（Se）的偏差与LDL-apoB（LDL颗粒数）和cmLDL呈正相关。
结论
在HC患者中，2种LDL-C均相检测在对LDL颗粒和cmLDL的反应性方面存在差异。直接测量反映修饰LDL的LDL-C可能是冠心病风险的更好标志物。
BACKGROUND
The aim of the present study was to compare 2 direct measurements for low-density lipoprotein cholesterol (LDL-C) with the Friedewald calculation (LDL-C [F]) in serum and their relationship with size-and charge-based LDL subfractions in serum ultracentrifugation fractions in patients with hypercholesterolemia (HC).
METHODS AND RESULTS
Serum samples from 283 HC patients who participated in a statin trial (the PATROL trial) were analyzed. Homogeneous assays for LDL-C were performed using reagents from Sekisui Medical (LDL-C [Se]) and Kyowa Medex (LDL-C [Ky]). Charge-based LDL subfractions were analyzed by capillary isotachophoresis (cITP). In whole serum in HC patients at baseline, LDL-C (Se) and LDL-C (Ky) negatively and positively deviated, respectively, from LDL-C (F). The negative deviation of LDL-C (Se) from LDL-C (F) increased with increasing LDL-C, while the positive deviation of LDL-C (Ky) from LDL-C (F) was positively correlated with charge-modified LDL (cmLDL) as analyzed by cITP. In serum d>1.006 g/ml and >1.040 g/ml fractions (LDL and small, dense LDL fractions, respectively), the deviation of LDL-C (Ky) from LDL-C (Se) was positively correlated with LDL-apoB (the number of LDL particles) and cmLDL.
CONCLUSIONS
The 2 homogenous assays for LDL-C differed with regard to reactivity toward LDL particles and cmLDL in patients with HC. Direct measurement of LDL-C that reflects modified LDL, could be a better marker for the risk of coronary heart disease.
影响因子：
5.3
作者：
Otokozawa S;Ai M;Asztalos BF;White CC;Demissie-Banjaw S;Cupples LA;Nakajima K;Wilson PW;Schaefer EJ
通讯作者：
Schaefer EJ