Double-Locking Mechanism of Self-Compatibility in Arabidopsis thaliana: The Synergistic Effect of Transcriptional Depression and Disruption of Coding Region in the Male Specificity Gene.
Double-Locking Mechanism of Self-Compatibility in Arabidopsis thaliana: The Synergistic Effect of Transcriptional Depression and Disruption of Coding Region in the Male Specificity Gene.
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拟南芥自交亲和性的双锁机制：雄性特异性基因编码区的转录抑制和破坏的协同效应。
DOI：
10.3389/fpls.2020.576140
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发表时间：
2020
影响因子：
5.6
通讯作者：
Watanabe M
中科院分区：
文献类型：
--
作者：
Suwabe K;Nagasaka K;Windari EA;Hoshiai C;Ota T;Takada M;Kitazumi A;Masuko-Suzuki H;Kagaya Y;Yano K;Tsuchimatsu T;Shimizu KK;Takayama S;Suzuki G;Watanabe M
拟南芥的自亲和性代表了相对较近的祖先专性异花授粉的中断，这被认为是开花植物中普遍存在的进化途径之一，正如达尔文所指出的。我们之前的研究发现，雄性特异性基因（SP11/SCR）的倒置会破坏自交不亲和，而这种自交不亲和可以通过反向表达SCR来恢复。然而，除了在启动子区和编码区发生关键反转外，拟南芥的SCR还存在其他突变，可能对转录调控产生影响。为了研究这些突变的功能后果，我们从自交亲和拟南芥和自交亲和拟南芥的同源位点中相互引入了原生启动子和下游序列。利用这对物种间基因对，我们可以将分析范围扩大到内含子的转录调控和缺失，以及原生基因组背景中的反转。初步分析表明，拟南芥在繁殖关键阶段SCR转录本的基础表达水平明显低于哈里氏拟南芥，表明该启动子在拟南芥中诱导转录的作用减弱。然而，在互惠转基因实验中，该拟南芥启动子能够恢复部分功能，如果与功能性拟南芥编码序列结合，尽管由于拟南芥的自兼容繁殖模式而发生了广泛的变化。这表明启动子和反转的协同作用导致了自相容性的固定，主要是通过SCR的破坏来实现的。我们的发现阐明了拟南芥历史转变的功能和进化背景，从而有助于理解导致自相容性发展的分子事件。
Self-compatibility in Arabidopsis thaliana represents the relatively recent disruption of ancestral obligate cross pollination, recognized as one of the prevalent evolutionary pathways in flowering plants, as noted by Darwin. Our previous study found that inversion of the male specificity gene (SP11/SCR) disrupted self-incompatibility, which was restored by overexpressing the SCR with the reversed inversion. However, SCR in A. thaliana has other mutations aside from the pivotal inversion, in both promoter and coding regions, with probable effects on transcriptional regulation. To examine the functional consequences of these mutations, we conducted reciprocal introductions of native promoters and downstream sequences from orthologous loci of self-compatible A. thaliana and self-incompatible A. halleri. Use of this inter-species pair enabled us to expand the scope of the analysis to transcriptional regulation and deletion in the intron, in addition to inversion in the native genomic background. Initial analysis revealed that A. thaliana has a significantly lower basal expression level of SCR transcripts in the critical reproductive stage compared to that of A. halleri, suggesting that the promoter was attenuated in inducing transcription in A. thaliana. However, in reciprocal transgenic experiments, this A. thaliana promoter was able to restore partial function if coupled with the functional A. halleri coding sequence, despite extensive alterations due to the self-compatible mode of reproduction in A. thaliana. This represents a synergistic effect of the promoter and the inversion resulting in fixation of self-compatibility, primarily enforced by disruption of SCR. Our findings elucidate the functional and evolutionary context of the historical transition in A. thaliana thus contributing to the understanding of the molecular events leading to development of self-compatibility.
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独立的S-locus突变导致拟南芥的自我育属性。
影响因子：
4.5
作者：
Boggs NA;Nasrallah JB;Nasrallah ME
通讯作者：
Nasrallah ME
影响因子：
4.9
作者：
Shimizu, Kentaro K.;Shimizu-Inatsugi, Rie;Purugganan, Michael D.
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Purugganan, Michael D.
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作者：
Kusaba, M;Dwyer, K;Nasrallah, ME
通讯作者：
Nasrallah, ME
影响因子：
4.9
作者：
Park, Jong-In;Ishimizu, Takeshi;Watanabe, Masao
通讯作者：
Watanabe, Masao
影响因子：
56.9
作者：
Nasrallah, ME;Liu, P;Nasrallah, JB
通讯作者：
Nasrallah, JB