Biodegradable lysine-derived polyurethane scaffolds promote healing in a porcine full-thickness excisional wound model.
Biodegradable lysine-derived polyurethane scaffolds promote healing in a porcine full-thickness excisional wound model.
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DOI：
10.1080/09205063.2014.965997
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Nanney LB
中科院分区：
文献类型：
--
作者：
Adolph EJ;Pollins AC;Cardwell NL;Davidson JM;Guelcher SA;Nanney LB
赖氨酸衍生的聚氨酯支架（LTI-PUR）支持皮肤松弛的小动物模型中的皮肤伤口愈合。由于人和猪皮肤的生理学和解剖学相似性，我们研究了LTI-PUR支架在猪切除伤口模型中支持伤口愈合的能力。对支架设计的修改包括添加羧甲基纤维素（CMC）作为致孔剂以增加互连性和额外的等离子体处理（等离子体）以降低表面疏水性。所有LTI-PUR支架和制剂都支持细胞浸润，并且是可生物降解的。在第15天，CMC和等离子体支架比LTI PUR或无处理更能模拟增加的巨噬细胞。这种反应与巨噬细胞介导的支架赖氨酸组分的氧化降解一致。在第8天和第15天，对照和支架处理的伤口中的细胞增殖相似。与未处理的伤口相比，在存在任何支架变化的情况下，细胞凋亡和血管面积密度均未显示出显著差异，这进一步证明了这些合成生物材料对这些关键伤口愈合过程没有不良影响。在全层猪切除伤口肉芽组织形成的关键阶段，LTI-PUR支架支持组织浸润，同时经历生物降解。对支架制造的修改修改修复过程。本研究强调了PUR支架制剂在临床相关动物模型中的生物相容性和有利的细胞反应。
Lysine-derived polyurethane scaffolds (LTI-PUR) support cutaneous wound healing in loose-skinned small animal models. Due to the physiological and anatomical similarities of human and pig skin we investigated the capacity of LTI-PUR scaffolds to support wound healing in a porcine excisional wound model. Modifications to scaffold design included the addition of carboxymethylcellulose (CMC) as a porogen to increase interconnectivity and an additional plasma treatment (Plasma) to decrease surface hydrophobicity. All LTI-PUR scaffold and formulations supported cellular infiltration and were biodegradable. At 15 days, CMC and Plasma scaffolds simulated increased macrophages more so than LTI PUR or no treatment. This response was consistent with macrophage-mediated oxidative degradation of the lysine component of the scaffolds. Cell proliferation was similar in control and scaffold treated wounds at 8 and 15 days. Neither apoptosis nor blood vessel area density showed significant differences in the presence of any of the scaffold variations compared to untreated wounds, providing further evidence that these synthetic biomaterials had no adverse effects on those pivotal wound healing processes. During the critical phase of granulation tissue formation in full thickness porcine excisional wounds, LTI-PUR scaffolds supported tissue infiltration, while undergoing biodegradation. Modifications to scaffold fabrication modify the reparative process. This study emphasizes the biocompatibility and favorable cellular responses of PUR scaffolding formulations in a clinically relevant animal model.
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影响因子：
4.9
作者：
Adolph, Elizabeth J.;Hafeman, Andrea E.;Davidson, Jeffrey M.;Nanney, Lillian B.;Guelcher, Scott A.
通讯作者：
Guelcher, Scott A.
影响因子：
1.4
作者：
Greenwood, John Edward;Dearman, Bronwyn Louise
通讯作者：
Dearman, Bronwyn Louise
影响因子：
4.6
作者：
Roy, Sashwati;Biswas, Sabyasachi;Sen, Chandan K.
通讯作者：
Sen, Chandan K.
DOI：
10.1083/jcb.119.3.493
发表时间：
1992-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gavrieli Y;Sherman Y;Ben-Sasson SA
通讯作者：
Ben-Sasson SA
影响因子：
6
作者：
Nanney, Lillian B.;Woodrell, Christopher D.;Gold, Leslie I.
通讯作者：
Gold, Leslie I.