Injectable polyurethane composite scaffolds delay wound contraction and support cellular infiltration and remodeling in rat excisional wounds.
Injectable polyurethane composite scaffolds delay wound contraction and support cellular infiltration and remodeling in rat excisional wounds.
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DOI：
10.1002/jbm.a.33266
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发表时间：
2012-02
影响因子：
4.9
通讯作者：
Guelcher, Scott A.
中科院分区：
文献类型：
--
作者：
Adolph, Elizabeth J.;Hafeman, Andrea E.;Davidson, Jeffrey M.;Nanney, Lillian B.;Guelcher, Scott A.
可注射支架因其能够填充不规则形状的缺陷和提供生长因子等生物物质而为伤口修复和再生提供了引人注目的机会。本研究采用大鼠皮肤切除模型，研究了可注射聚氨酯生物复合支架的性能及其在皮肤创面修复中的应用。该支架具有最小的反应放热和临床相关的工作和凝结时间。此外，生物复合材料具有与橡胶弹性体一致的力学和热性能。在大鼠切除创面模型中，在早期时间点注射可固定的生物复合支架支架，导致后期创面再生而不是瘢痕形成。伤口宽度和厚度的测量显示，与空白伤口相比，处理后的伤口在第7天收缩较少。细胞增殖和细胞凋亡分析表明，该支架具有良好的生物相容性，并支持组织的生长。肌成纤维细胞的形成和胶原纤维的组织提供了证据，表明支架在升高的张力下通过破坏排列的基质的形成而对细胞外基质的重塑具有积极的作用。综上所述，我们开发了一种可注射的可生物降解的聚氨酯生物复合材料支架，可以促进大鼠皮肤伤口的愈合。
Injectable scaffolds present compelling opportunities for wound repair and regeneration due to their ability to fill irregularly shaped defects and deliver biologics such as growth factors. In this study, we investigated the properties of injectable polyurethane biocomposite scaffolds and their application in cutaneous wound repair using a rat excisional model. The scaffolds have a minimal reaction exotherm and clinically relevant working and setting times. Moreover, the biocomposites have mechanical and thermal properties consistent with rubbery elastomers. In the rat excisional wound model, injection of settable biocomposite scaffolds stented the wounds at early time points, resulting in a regenerative rather than a scarring phenotype at later time points. Measurements of wound width and thickness revealed that the treated wounds were less contracted at day 7 compared to blank wounds. Analysis of cell proliferation and apoptosis showed that the scaffolds were biocompatible and supported tissue ingrowth. Myofibroblast formation and collagen fiber organization provided evidence that the scaffolds have a positive effect on extracellular matrix remodeling by disrupting the formation of an aligned matrix under elevated tension. In summary, we have developed an injectable biodegradable polyurethane biocomposite scaffold that enhances cutaneous wound healing in a rat model.
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影响因子：
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