Sp1-Induced lncRNA Rmrp Promotes Mesangial Cell Proliferation and Fibrosis in Diabetic Nephropathy by Modulating the miR-1a-3p/JunD Pathway.
Sp1-Induced lncRNA Rmrp Promotes Mesangial Cell Proliferation and Fibrosis in Diabetic Nephropathy by Modulating the miR-1a-3p/JunD Pathway.
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Sp1诱导的lncRNA Rmrp通过调节miR-1a-3p/JunD通路促进糖尿病肾病肾小球系膜细胞增殖和纤维化
DOI：
10.3389/fendo.2021.690784
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发表时间：
2021
影响因子：
5.2
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Yang H;Wang J;Zhang Z;Peng R;Lv D;Liu H;Sun Y
Diabetic nephropathy (DN) is a serious complication of diabetes mellitus. Long non-coding RNAs (lncRNAs) are regulators in DN progression. However, the regulatory mechanisms of multiple lncRNAs in DN remain to be determined. Our aim was to investigate the function and molecular mechanism of lncRNA RNA component of mitochondrial RNAase P (Rmrp) in DN. Here, we observed that the expression of Rmrp was up-regulated in the kidney of db/db DN mice and high glucose induced glomerular mesangial cells (MC). More importantly, the abnormal transcription of Rmrp was induced by nuclear transcription factor Sp1, which promotes the proliferation and production of fibrotic markers in MC. Subsequently, we screened the miRNAs related to Rmrp and found that Rmrp and miR-1a-3p are co-localized at the subcellular level of MC, and Rmrp could directly binds to miR-1a-3p. Further mechanism research demonstrated that the elevated miR-1a-3p significantly attenuated the proliferation and fibrosis-promoting effects induced by up-regulation of Rmrp. At the same time, we also investigated that miR-1a-3p can directly bind to Jun D proto-oncogene (JunD), thereby regulating the protein level of JunD. Rmrp-induced proliferation and fibrogenesis were reversed by co-transfection with JunD siRNA. In summary, Sp1 induced lncRNA Rmrp could drive the expression of JunD via sponging miR-1a-3p in DN progression.
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影响因子：
12.3
作者：
Marchese FP;Raimondi I;Huarte M
通讯作者：
Huarte M
长非编码RNA协调线粒体和细胞核之间的功能
影响因子：
3.9
作者：
Dong Y;Yoshitomi T;Hu JF;Cui J
通讯作者：
Cui J
DOI：
10.26355/eurrev_202003_20545
发表时间：
2020-01-01
影响因子：
3.3
作者：
An, J-H;Chen, Z-Y;Shi, F-W
通讯作者：
Shi, F-W
影响因子：
6.6
作者：
Bai, Xiaoyan;Geng, Jian;Liu, Xiaoting
通讯作者：
Liu, Xiaoting
影响因子：
9.7
作者：
Elliott, Bethtrice;Millena, Ana Cecilia;Khan, Shafiq
通讯作者：
Khan, Shafiq