Marker-assisted selection for pyramiding the waxy and opaque-16 genes in maize using cross and backcross schemes
Marker-assisted selection for pyramiding the waxy and opaque-16 genes in maize using cross and backcross schemes
复制标题
使用杂交和回交方案进行玉米中蜡质和不透明 16 基因金字塔化的标记辅助选择
DOI：
10.1007/s11032-012-9830-8
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发表时间：
2013-01
影响因子：
3.1
通讯作者：
Wang, Mingchun
中科院分区：
文献类型：
--
作者：
Yang, Liuqi;Wang, Wei;Yang, Wenpeng;Wang, Mingchun
玉米中的蜡质（wx）基因与胚乳中较高的支链淀粉含量和更好的风味有关。 opaque-16 (o16) 基因与胚乳中较高的赖氨酸含量和更好的营养价值相关。为了聚合 wx 和 o16 基因，使用 o16 品系 QCL3024 和两个蜡质品系 QCL5019 和 QCL5008 作为亲本构建了杂交和回交群体。使用o16基因的连锁标记umc1141和wx基因的内部标记phi027来选择目标基因。覆盖整个基因组的简单序列重复标记用于回交群体个体后代的背景选择。使用 Acid Orange-12 染料结合赖氨酸方法测定谷物赖氨酸含量。分别使用 I2-KI 程序和双波长分光光度法对支链淀粉的谷物含量进行定性和定量分析。从杂交群体的F4代获得双隐性基因型wxwxo16o16的4个品系，从回交群体的BC2F4代获得3个相同基因型的品系。金字塔系的赖氨酸含量分别比蜡质亲本 QCL5019 和 QCL5008 高 16-27% 和 18-28%。金字塔系的支链淀粉含量比高赖氨酸亲本 QCL3024 多 61-63%。回交群体的三个金字塔品系与蜡质亲本 QCL5008 具有相似的遗传背景。我们的研究结果对于提高玉米品质具有重要意义。
The waxy (wx) gene in maize is associated with higher content of amylopectin in the endosperm and better flavor. The opaque-16 (o16) gene is associated with higher lysine content in the endosperm and better nutritional value. To pyramid the wx and o16 genes, cross and backcross populations were constructed using the o16 line QCL3024 and the two waxy lines, QCL5019 and QCL5008, as parents. The linkage marker umc1141 for the o16 gene and the internal marker phi027 for the wx gene were used to select the target genes. Simple sequence repeat markers covering the whole genome were used for background selection in individual progenies of the backcross population. The grain lysine content was determined using the Acid Orange-12 Dye Binding Lysine method. Qualitative and quantitative analyses of the grain content of amylopectin were performed using the I2-KI procedure and double-wavelength spectrophotometry, respectively. Four lines of the double recessive genotype wxwxo16o16 were obtained from the F4 generation of the cross population and three lines of the same genotype were obtained from the BC2F4 generation of the backcross population. The lysine content of the pyramid lines was 16–27 and 18–28 % higher than the waxy parents QCL5019 and QCL5008, respectively. The pyramid lines had 61–63 % more amylopectin than the high-lysine parent QCL3024. The three pyramid lines from the backcross population had similar genetic background to the waxy parent QCL5008. Our results are of significance for the improvement of maize quality.
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影响因子：
3.3
作者：
F. Hospital;C. Chevalet;P. Mulsant
通讯作者：
F. Hospital;C. Chevalet;P. Mulsant
DOI：
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发表时间：
1974
期刊：
--
影响因子：
--
作者：
P. Mangelsdorf
通讯作者：
P. Mangelsdorf
影响因子：
1.9
作者：
C. Stonor;E. Anderson
通讯作者：
C. Stonor;E. Anderson
影响因子：
48
作者：
G. Collins
通讯作者：
G. Collins
DOI：
10.5962/bhl.title.108096
发表时间：
2012-01
期刊：
--
影响因子：
--
作者：
J. Kempton
通讯作者：
J. Kempton