The miR-221/222 cluster, miR-10b and miR-92a are highly upregulated in metastatic minimally invasive follicular thyroid carcinoma.
The miR-221/222 cluster, miR-10b and miR-92a are highly upregulated in metastatic minimally invasive follicular thyroid carcinoma.
复制标题
DOI：
10.3892/ijo.2013.1879
复制
发表时间：
2013-06
影响因子：
5.2
通讯作者：
Takizawa T
中科院分区：
文献类型：
--
作者：
Jikuzono T;Kawamoto M;Yoshitake H;Kikuchi K;Akasu H;Ishikawa H;Hirokawa M;Miyauchi A;Tsuchiya S;Shimizu K;Takizawa T
微创甲状腺滤泡癌（MI-FTC）的特点是包膜和/或血管浸润有限，长期预后良好。然而，一些MI-FTC病例由于严重的远处转移而显示出不良预后（即，转移性MI-FTC）。尽管如此，还没有建立方法来预测MI-FTC的预后。本研究旨在通过使用microRNA（miRNA）鉴定转移性MI-FTC的新预后因素。34例MI-FTC患者分为两组：转移性组，M（+）（n=12）和非转移性组，M（-）（n=22）。在M（+）组中，在首次手术确定MI-FTC诊断后发现远处转移。在M（-）组中，术后≥10年未发现远处转移。使用激光显微切割，然后进行定量实时PCR和PCR阵列，我们进行了一个全面的表达谱的667 miRNA的福尔马林固定，石蜡包埋的样品从最初的MI-FTC操作。此外，我们通过logistic回归分析评估了miRNA作为MI-FTC转移潜力的新型生物标志物的潜在用途。MI-FTC样品中miRNA表达的综合定量分析显示，miR-221/222簇（即，miR-221、miR-222和miR-222*）、miR-10 b和miR-92 a在M（+）组中的表达显著高于M（-）组。有趣的是，这些miRNA的表达水平在具有远处转移和更差预后的广泛侵袭性FTC（WI-FTC; n=13）中也显示上调，这表明转移性MI-FTC和WI-FTC之间的miRNA表达密切相似。Logistic回归分析显示miR-10 b对预后有显著影响（OR 19.759，95%CI 1.433-272.355，p= 0.026）。我们的研究结果表明，miR-10 b是一个潜在的预后因素，评估转移潜力的MI-FTC在初始操作阶段。
Minimally invasive follicular thyroid carcinoma (MI-FTC) is characterized by limited capsular and/or vascular invasion with good long-term outcomes. However, some cases of MI-FTC show a poor prognosis because of severe distant metastasis (i.e., metastatic MI-FTC). Nonetheless, no method has been established for predicting the prognosis of MI-FTC. This study was conducted to identify novel prognostic factors for metastatic MI-FTC by the use of microRNA (miRNA). Thirty-four patients with MI-FTC were categorized into two groups: the metastatic group, M(+) (n=12) and the non-metastatic group, M(−) (n=22). In the M(+) group, distant metastasis was recognized after the initial operation established the diagnosis of MI-FTC. In the M(−) group, no distant metastasis was recognized postoperatively for ≥10 years. Using laser micro-dissection followed by quantitative real-time PCR and PCR arrays, we performed a comprehensive expression profiling of 667 miRNAs in formalin-fixed, paraffin-embedded samples from the initial MI-FTC operation. Furthermore, we assessed the potential use of miRNAs as novel biomarkers for the metastatic potential of MI-FTC by logistic regression analysis. Comprehensive quantitative analysis of miRNA expression in MI-FTC samples revealed that the miR-221/222 cluster (i.e., miR-221, miR-222 and miR-222*), miR-10b and miR-92a were significantly upregulated in the M(+) group compared with the M(−) group. Interestingly, the expression levels of these miRNAs were also shown to be upregulated in widely invasive FTC (WI-FTC; n=13) that has distant metastasis and worse prognosis, indicating a close similarity in the miRNA expression between metastatic MI-FTC and WI-FTC. Logistic regression analysis revealed that miR-10b made a significant contribution to prognosis (OR 19.759, 95% CI 1.433–272.355, p= 0.026). Our findings suggest that miR-10b is a potential prognostic factor for evaluating the metastatic potential of MI-FTC at an initial operation stage.
登录
查看更多内容
影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
3.9
作者：
Foukakis, Theodoros;Gusnanto, Arief;Zedenius, Jan
通讯作者：
Zedenius, Jan
影响因子：
9
作者：
Asari, Reza;Koperek, Oskar;Niederle, Bruno
通讯作者：
Niederle, Bruno
影响因子：
8.8
作者：
Diosdado B;van de Wiel MA;Terhaar Sive Droste JS;Mongera S;Postma C;Meijerink WJ;Carvalho B;Meijer GA
通讯作者：
Meijer GA
影响因子：
11.2
作者：
Iorio, MV;Ferracin, M;Croce, CM
通讯作者：
Croce, CM