Open-source computational framework for studying Drosophila behavioral phase.
Open-source computational framework for studying Drosophila behavioral phase.
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DOI：
10.1016/j.xpro.2020.100285
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Fernandez MP
中科院分区：
文献类型：
--
作者：
Kostadinov B;Lee Pettibone H;Bell EV;Zhou X;Pranevicius A;Shafer OT;Fernandez MP
This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experiments. We also provide an open-source computational framework using R for the analysis of the phase of behavior using circular statistics. An extended method for complete use is also provided. For complete details on the use and execution of this protocol, please refer to. R framework for analysis of Drosophila behavioral phase and circular statistics analysis Visualization of activity, phase of behavior, and rose plots This protocol describes a standardized method for analyzing Drosophila behavioral rhythmicity under light dark cycles, temperature ramps, and free running conditions. The protocol constitutes a step-by-step guide from generation of appropriate Drosophila genetic crosses to behavioral experiments. We also provide an open-source computational framework using R for the analysis of the phase of behavior using circular statistics. An extended method for complete use is also provided.
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