Use of adipose-derived stem cells to fabricate scaffoldless tissue-engineered neural conduits in vitro.
Use of adipose-derived stem cells to fabricate scaffoldless tissue-engineered neural conduits in vitro.
复制标题
DOI：
10.1016/j.neuroscience.2011.11.004
复制
发表时间：
2012-01-10
期刊：
影响因子：
3.3
通讯作者：
Larkin, L. M.
中科院分区：
文献类型：
--
作者：
Adams, A. M.;Arruda, E. M.;Larkin, L. M.
由创伤或疾病引起的周围神经损伤通常需要手术干预。虽然这种修复的黄金标准是利用自体神经移植物，但目前正在设计和评估不需要侵入性采集自体神经组织的替代方案。我们之前建立了使用由原代细胞制造的无支架工程神经导管作为大鼠坐骨神经修复的替代方案（;亚当斯2011）。本研究建立了从分化为成纤维细胞或神经谱系的脂肪源性干细胞（ASC）制造神经导管的方案，并共培养成三维无支架组织工程神经导管。添加抗坏血酸-2-磷酸和成纤维细胞生长因子FGF-2的培养基诱导和分化的ASC成纤维细胞谱系中超过90%的细胞群体，证实了胶原蛋白I的表达。ASC分化的成纤维细胞形成单层，分层并形成3-D管道。通过在添加表皮生长因子EGF和FGF-2的无血清neurobasal培养基中的非粘附表面上培养ASC形成神经球。添加10 ng EGF和10 ng FGF-2比低EGF和FGF-2浓度的治疗产生更大和更多的神经球。S100的表达证实了随后向胶质样细胞的分化。将成纤维细胞单层与神经球共培养，构建无支架的三维组织工程神经导管。它们的神经样结构和与再生轴突相关的神经胶质样细胞的掺入可能使这些新的干细胞衍生的神经导管成为修复周围神经损伤后的关键间隙的有效技术。
Peripheral nerve injuries resulting from trauma or disease often necessitate surgical intervention. While the gold standard for such repairs utilizes nerve autografts, alternatives that do not require invasive harvesting of autologous nerve tissues are currently being designed and evaluated. We previously established the use of scaffold-less engineered neural conduits fabricated from primary cells as one such alternative in sciatic nerve repair in rats (; Adams 2011). The present study establishes protocols for fabricating neural conduits from adipose-derived stem cells (ASCs) differentiated to either a fibroblast or neural lineage and co-cultured into a three-dimensional scaffold-less tissue engineered neural conduit. Addition of ascorbic acid-2-phosphate and fibroblast growth factor FGF-2 to the medium induced and differentiated ASCs to a fibroblast lineage in over 90% of the cell population, as confirmed by collagen I expression. ASC-differentiated fibroblasts formed monolayers, delaminated and formed 3-D conduits. Neurospheres were formed by culturing ASCs on non-adherent surfaces in serum-free neurobasal medium with the addition of epidermal growth factor EGF and FGF-2. The addition of 10 ng EGF and 10 ng FGF-2 produced larger and more numerous neurospheres than treatments of lower EGF and FGF-2 concentrations. Subsequent differentiation to glial-like cells was confirmed by the expression of S100. ASC-derived fibroblast monolayers and neurospheres were co-cultured to fabricate a three-dimensional scaffold-less tissue engineered neural conduit. Their nerve-like structure and incorporation of glial-like cells which would associate with regenerating axons may make these novel, stem cell derived neural conduits an efficacious technology for repairing critical gaps following peripheral nerve injury.
登录
查看更多内容
影响因子：
4.6
作者：
Yamamoto, Naoki;Akamatsu, Hirohiko;Matsunaga, Kayoko
通讯作者：
Matsunaga, Kayoko
影响因子：
1.4
作者：
Shokouhi, Ghaffar;Tubbs, R. Shane;Oakes, W. Jerry
通讯作者：
Oakes, W. Jerry
DOI：
10.1197/j.jht.2005.02.012
发表时间：
2005-04-01
期刊：
Journal of hand therapy : official journal of the American Society of Hand Therapists
影响因子：
--
作者：
Taras, John S;Nanavati, Vipul;Steelman, Pamela
通讯作者：
Steelman, Pamela
影响因子：
2.1
作者：
Baltich, Jennifer;Hatch-Vallier, Leah;Adams, Aaron M.;Arruda, Ellen M.;Larkin, Lisa M.
通讯作者：
Larkin, Lisa M.
影响因子：
3.3
作者：
di Summa, P. G.;Kalbermatten, D. F.;Terenghi, G.
通讯作者：
Terenghi, G.