Differential miRNA Profiles Correlate With Disparate Immunity Outcomes Associated With Vaccine Immunization and Chlamydial Infection.
Differential miRNA Profiles Correlate With Disparate Immunity Outcomes Associated With Vaccine Immunization and Chlamydial Infection.
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差异miRNA谱与疫苗免疫和衣原体感染相关的不同免疫结果相关。
DOI：
10.3389/fimmu.2021.625318
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发表时间：
2021
影响因子：
7.3
通讯作者：
Eko FO
中科院分区：
文献类型：
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作者：
Howard S;Richardson S;Benyeogor I;Omosun Y;Dye K;Medhavi F;Lundy S;Adebayo O;Igietseme JU;Eko FO
有希望的衣原体疫苗免疫后疫苗诱导的免疫反应保护实验动物免受衣原体引起的上生殖道病变和不孕症的影响。相比之下，原始生殖器感染活的衣原体并不能预防这些病理。我们假设，在小鼠的上生殖道(UGT)诱导的不同miRNA谱与疫苗免疫和衣原体感染相关的不同免疫和病理结果相关。因此，本研究采用纳米串小鼠miRNA分析方法，比较了衣原体感染(Live EB)和树突状细胞(DC)疫苗(DC疫苗)和VCG疫苗(VCG疫苗)免疫后小鼠UGT中miRNA的表达谱。在免疫和感染小鼠UGT中差异表达的602个miRNAs中，我们选择了58个计数为>；100和p值为0.05的miRNAs进行进一步分析。有趣的是，疫苗免疫和衣原体感染诱导了不同的miRNA谱的表达，与衣原体感染的小鼠相比，疫苗免疫的小鼠的比例更高；DC疫苗(41只)、VCG疫苗(23只)和Live EB(15只)。系统聚类分析显示，每个实验组独特的DE miRNAs存在显著差异，DC疫苗显示最多(21个上调，5个下调)，VCG疫苗(2个上调，5个下调)和活体EB(2个上调，4个下调)。DC疫苗免疫组的表达最多(21个上调，5个下调，而活衣原体感染组中有2个上调，4个下调)。通路分析表明，DE miRNAs的目标基因调控与免疫反应和炎症相关的几个生物学过程和功能。这些结果表明，诱导不同的miRNA表达在衣原体感染和疫苗接种相关的不同免疫结果中起着重要作用。
Vaccine-induced immune responses following immunization with promising Chlamydia vaccines protected experimental animals from Chlamydia-induced upper genital tract pathologies and infertility. In contrast, primary genital infection with live Chlamydia does not protect against these pathologies. We hypothesized that differential miRNA profiles induced in the upper genital tracts (UGT) of mice correlate with the disparate immunity vs. pathologic outcomes associated with vaccine immunization and chlamydial infection. Thus, miRNA expression profiles in the UGT of mice after Chlamydia infection (Live EB) and immunization with dendritic cell (DC)-based vaccine (DC vaccine) or VCG-based vaccine (VCG vaccine) were compared using the NanoString nCounter Mouse miRNA assay. Of the 602 miRNAs differentially expressed (DE) in the UGT of immunized and infected mice, we selected 58 with counts >100 and p-values < 0.05 for further analysis. Interestingly, vaccine immunization and Chlamydia infection induced the expression of distinct miRNA profiles with a higher proportion in vaccine-immunized compared to Chlamydia infected mice; DC vaccine (41), VCG vaccine (23), and Live EB (15). Hierarchical clustering analysis showed notable differences in the uniquely DE miRNAs for each experimental group, with DC vaccine showing the highest number (21 up-regulated, five down-regulated), VCG vaccine (two up-regulated, five down-regulated), and live EB (two up-regulated, four down-regulated). The DC vaccine-immunized group showed the highest number (21 up-regulated and five down-regulated compared to two up-regulated and four down-regulated in the live Chlamydia infected group). Pathway analysis showed that the DE miRNAs target genes that regulate several biological processes and functions associated with immune response and inflammation. These results suggest that the induction of differential miRNA expression plays a significant role in the disparate immunity outcomes associated with Chlamydia infection and vaccination.
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影响因子：
--
作者：
Gupta R;Arkatkar T;Keck J;Koundinya GK;Castillo K;Hobel S;Chambers JP;Yu JJ;Guentzel MN;Aigner A;Christenson LK;Arulanandam BP
通讯作者：
Arulanandam BP
影响因子：
2.3
作者：
Guo J;Guan Q;Liu X;Wang H;Gleave ME;Nguan CY;Du C
通讯作者：
Du C
MicroRNA-200C的表达水平通过调节胃癌中的Zeb1/2和E-钙粘着蛋白的调节与细胞形态和组织学分化有关。
影响因子：
4.2
作者：
Kurata A;Yamada M;Ohno SI;Inoue S;Hashimoto H;Fujita K;Takanashi M;Kuroda M
通讯作者：
Kuroda M
影响因子：
3.7
作者：
Derrick T;Last AR;Burr SE;Roberts CH;Nabicassa M;Cassama E;Bailey RL;Mabey DC;Burton MJ;Holland MJ
通讯作者：
Holland MJ
影响因子：
17.1
作者：
Eming SA;Martin P;Tomic-Canic M
通讯作者：
Tomic-Canic M