Anticancer activity of guava ( Psidium guajava L.) branch extracts against HT-29 human colon cancer cells
Anticancer activity of guava ( Psidium guajava L.) branch extracts against HT-29 human colon cancer cells
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番石榴 (Psidium guajava L.) 分支提取物对 HT-29 人结肠癌细胞的抗癌活性
DOI：
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Park HaeRyong
中科院分区：
文献类型：
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作者：
Lee Sangbong;Park HaeRyong
天然产物由于其潜在的药理活性，近年来已成为研究热点。因此，我们建立了一个程序来筛选天然产物的细胞毒活性使用MTT还原测定系统测试HT-29人结肠癌细胞。在筛选过程中，我们发现番石榴（Psidium guajava L.）分支（GBA）对HT-29细胞有明显的细胞毒作用。MTT还原试验、LDH释放试验和集落形成试验显示GBA具有很强的细胞毒作用。特别是250 μg/ml的GBA对HT-29细胞的生长抑制率为35.5%。正如预期的那样，GBA在HT-29细胞中诱导了特征性的凋亡效应，包括在250 μg/ml的浓度水平下处理细胞24 h后发生的染色质浓缩和鲨鱼化。为了检测细胞凋亡的功能，我们使用流式细胞仪分析。亚G1期DNA含量显示凋亡细胞的分布与细胞周期相一致。
Natural products have recently become the focus of increased research interest due to their potential pharmacological activities. Therefore, we established a program to screen natural products for cytotoxic activity using the MTT reduction assay system to test HT-29 human colon cancer cells. During the course of screening, we found that the acetone extracts of guava (Psidium guajava L.) branch (GBA) had cytotoxic effects on HT-29 cells. The GBA showed highly cytotoxic effects via the MTT reduction assay, LDH release assay, and colony formation assay. In particular, the GBA of the 250 μg/ml showed 35.5% inhibition against growth of HT-29 cells. As expected, GBA induced characteristic apoptotic effects in HT-29 cells, including chromatin condensation and sharking that occurred 24 h after the cells had been treated at a concentration level of 250 μg/ml. To examine the functions on apoptosis, we used a flow cytometric analysis. The apoptotic cells were distributed according to the cell cycle phase shown by sub-G1 DNA content.
影响因子：
9.7
作者：
Saleem, Mohammad
通讯作者：
Saleem, Mohammad