Prolonged exposure to isoflurane ameliorates infarction severity in the rat pup model of neonatal hypoxia-ischemia.
Prolonged exposure to isoflurane ameliorates infarction severity in the rat pup model of neonatal hypoxia-ischemia.
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DOI：
10.1007/s12975-011-0081-5
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发表时间：
2011-09-01
影响因子：
6.9
通讯作者：
Tang, Jiping
中科院分区：
文献类型：
--
作者：
Chen, Hank;Burris, Michael;Fajilan, Adrain;Spagnoli, Fred;Zhang, John H.;Tang, Jiping
新生儿缺氧缺血大鼠模型被称为Rice-Vannucci模型，广泛用于研究围产期缺氧缺血和儿童脑损伤。该模型的主要缺点之一是动物脑梗死的不一致性。我们推测，梗死的不一致性是由于手术时间延长，因此异氟烷暴露。新生儿缺氧缺血诱导出生后第7和10天的大鼠幼仔单侧右颈总动脉结扎，然后缺氧2.5小时（8%的氧气）。切开至结扎（ITL）定义为从初始切开（2%异氟烷暴露后4分钟）至完成颈动脉结扎（此时也终止异氟烷暴露）的时间量。在研究的第一部分中，将每组的ITL指定为5、13和21 min。在研究的第二部分中，将ITL指定为4 min;然而，使用持续异氟烷使每组暴露于异氟烷5、13和21 min。在术后48 h评估脑梗死的预后。通过旋转棒试验评估运动缺陷。ITL 5 min组脑梗死明显，ITL 13、21 min组脑梗死明显减少（P<0.05）。在研究的第二部分中，在5分钟异氟烷暴露组中观察到明显的脑梗死，并且在13分钟和21分钟异氟烷暴露组中均观察到脑梗死减少（P<0.05）。转棒试验与5 min ITL组相似，5 min异氟醚组的转棒试验下降更明显（P<0.05）。本研究表明，新生缺氧缺血大鼠模型脑梗死不一致性与手术时间有关。观察到的脑梗死的时间依赖性降低与异氟烷暴露时间相关。较短的手术时间和异氟醚暴露改善了脑梗死和运动障碍的模型一致性。
The neonatal hypoxia-ischemia rat model referred to as the Rice–Vannucci model is extensively used to study perinatal hypoxia-ischemia and child brain injury. One of the major weaknesses of this model is its inconsistency of brain infarction among animals. We hypothesize that the inconsistency of infarction is caused by prolonged operation time and therefore isoflurane exposure. Neonatal hypoxia-ischemia was induced in postnatal days 7 and 10 rat pups by unilateral right common carotid ligation followed by 2.5 h of hypoxia (8% oxygen). The incision-to-ligation (ITL) was defined as the amount of time from initial incision (4 min after 2% isoflurane exposure) to completion of carotid ligation (at which point isoflurane exposure was also terminated). In the first part of the study, the ITL of each group was designated to be 5, 13, and 21 min. In the second part of the study, the ITL is designated to 4 min; however, continued isoflurane was used to make 5, 13, and 21 min isoflurane exposure for each group. Percentages of brain infarction were assessed at 48 h following surgery. Motor deficits were accessed by Rotarod test. Marked brain infarction was observed in the 5-min ITL group and a decrease of brain infarction observed in the 13-and 21-min groups (P<0.05). In the second part of the study, marked brain infarction was observed in the 5-min isoflurane exposure group, and a decrease of brain infarction was observed in each of the 13- and 21-min isoflurane exposure groups (P<0.05). Similar tendencies were observed in Rotarod tests than 5-min ITL and 5-min isoflurane groups showed more marked deficits (P<0.05). This study demonstrated that brain infarction inconsistency of the neonatal hypoxia-ischemia rat pup model is related to the operation time. The observed time-dependent decrease of brain infarction is correlated to the isoflurane exposure time. Shorter operation and isoflurane exposure improves this model consistency of brain infarction and motor deficits.
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影响因子：
4.2
作者：
HAMM, RJ;PIKE, BR;JENKINS, LW
通讯作者：
JENKINS, LW
影响因子：
4.7
作者：
DALKARA, T;YOSHIDA, T;MOSKOWITZ, MA
通讯作者：
MOSKOWITZ, MA
影响因子：
8.3
作者：
Bhardwaj, A;Castro, AF;Kirsch, JR
通讯作者：
Kirsch, JR
影响因子：
56.9
作者：
HUANG, ZH;HUANG, PL;MOSKOWITZ, MA
通讯作者：
MOSKOWITZ, MA
影响因子：
8.3
作者：
Kapinya, KJ;Löwl, D;Dirnagl, U
通讯作者：
Dirnagl, U