Neural correlates of winning and losing fights in poison frog tadpoles
Neural correlates of winning and losing fights in poison frog tadpoles
复制标题
毒蛙蝌蚪战斗胜利和失败的神经相关性
DOI：
10.1101/2020.01.27.922286
复制
发表时间：
2020
影响因子：
2.9
通讯作者：
L. O’Connell
中科院分区：
文献类型：
--
作者：
E. K. Fischer;Harmony Alvarez;Katherine M. Lagerstrom;Jordan E. McKinney;Randi Petrillo;Gwen Ellis;L. O’Connell
在许多物种中，都有记录表明幼年动物之间存在对资源的侵略性竞争，但对调节幼年动物这种行为的神经机制却知之甚少。在毒蛙中，增加父母的照顾与蝌蚪池的水量减少，资源限制和侵略性有关。事实上，许多种毒蛙的蝌蚪会攻击、杀死和吃掉其他蝌蚪。我们研究了神经基础的同种侵略染色毒蛙（Dendrobates tinctorius）蝌蚪通过比较个人赢得了侵略性的遭遇，失去了侵略性的遭遇，或没有从事战斗。我们首先使用磷酸化核糖体（pS6）的免疫组织化学检测作为与行为相关的神经激活的代理比较了广义神经活动的模式。我们发现，增加的神经活动在内侧腭和视前区的失败者蝌蚪，这表明哺乳动物海马和视前区的两栖类同源物可能有助于失败者相关的行为。在其他脊椎动物中，九肽（精氨酸催产素和中催产素）和多巴胺与攻击性有关，位于视前区。接下来，我们使用双标记免疫组织化学技术检测了九肽和酪氨酸羟化酶阳性细胞的神经活性。我们发现增加的神经活动，特别是在视前区的九肽神经元的赢家，而我们没有发现行为组之间的多巴胺能细胞的活动差异。我们的研究结果表明，神经相关的毒蛙蝌蚪的侵略性是类似的神经机制介导的其他脊椎动物类群的成人和青少年的侵略。
Aggressive competition for resources among juveniles is documented in many species, but the neural mechanisms regulating this behavior in young animals are poorly understood. In poison frogs, increased parental care is associated with decreased water volume of tadpole pools, resource limitation, and aggression. Indeed, the tadpoles of many poison frog species will attack, kill, and cannibalize other tadpoles. We examined the neural basis of conspecific aggression in Dyeing poison frog (Dendrobates tinctorius) tadpoles by comparing individuals that won aggressive encounters, lost aggressive encounters, or did not engage in a fight. We first compared patterns of generalized neural activity using immunohistochemical detection of phosphorylated ribosomes (pS6) as a proxy for neural activation associated with behavior. We found increased neural activity in the medial pallium and preoptic area of loser tadpoles, suggesting the amphibian homologs of the mammalian hippocampus and preoptic area may facilitate loser-associated behaviors. Nonapeptides (arginine vasotocin and mesotocin) and dopamine have been linked to aggression in other vertebrates and are located in the preoptic area. We next examined neural activity specifically in nonapeptide- and tyrosine-hydroxylase-positive cells using double-label immunohistochemistry. We found increased neural activity specifically in the preoptic area nonapeptide neurons of winners, whereas we found no differences in activity of dopaminergic cells among behavioral groups. Our findings suggest the neural correlates of aggression in poison frog tadpoles are similar to neural mechanisms mediating aggression in adults and juveniles of other vertebrate taxa.
影响因子：
4.2
作者：
Thiel, Kenneth J.;Okun, Alec C.;Neisewander, Janet L.
通讯作者：
Neisewander, Janet L.