The Photosystem II Assembly Factor Ycf48 from the Cyanobacterium Synechocystis sp. PCC 6803 Is Lipidated Using an Atypical Lipobox Sequence.
The Photosystem II Assembly Factor Ycf48 from the Cyanobacterium Synechocystis sp. PCC 6803 Is Lipidated Using an Atypical Lipobox Sequence.
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DOI：
10.3390/ijms22073733
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发表时间：
2021-04-02
影响因子：
5.6
通讯作者：
Komenda J
中科院分区：
文献类型：
--
作者：
Knoppová J;Yu J;Janouškovec J;Halada P;Nixon PJ;Whitelegge JP;Komenda J
光合作用过程中的光化学能量转换是由膜包埋的叶绿素结合蛋白复合物完成的。这些复合物的生物发生和维持需要辅助蛋白因子，以优化组装过程并保护新生复合物免受光损伤。在蓝藻中，几种脂蛋白有助于光系统II（PSII）复合体的生物发生和功能。它们包括CyanoP、CyanoQ和Psb 27，它们都连接到PSII复合物的内腔侧。在这里，我们表明，在蓝藻集胞藻属PCC 6803中发现的内腔Ycf 48组装因子也是一种脂蛋白。由定点诱变实验支持的分离的蛋白质的详细质谱分析表明Ycf 48的N-末端C29残基的脂化和从C-末端去除三个氨基酸。Ycf 48中的脂盒序列在−3位含有一个半胱氨酸残基，而在典型的脂盒序列中则含有Leu/瓦尔/Ile残基。非典型Ycf 48脂盒序列存在于大多数蓝藻中，但在真核生物中不存在。脂蛋白在蓝藻PSII的协调装配中可能发挥的作用进行了讨论。
Photochemical energy conversion during oxygenic photosynthesis is performed by membrane-embedded chlorophyll-binding protein complexes. The biogenesis and maintenance of these complexes requires auxiliary protein factors that optimize the assembly process and protect nascent complexes from photodamage. In cyanobacteria, several lipoproteins contribute to the biogenesis and function of the photosystem II (PSII) complex. They include CyanoP, CyanoQ, and Psb27, which are all attached to the lumenal side of PSII complexes. Here, we show that the lumenal Ycf48 assembly factor found in the cyanobacterium Synechocystis sp. PCC 6803 is also a lipoprotein. Detailed mass spectrometric analysis of the isolated protein supported by site-directed mutagenesis experiments indicates lipidation of the N-terminal C29 residue of Ycf48 and removal of three amino acids from the C-terminus. The lipobox sequence in Ycf48 contains a cysteine residue at the −3 position compared to Leu/Val/Ile residues found in the canonical lipobox sequence. The atypical Ycf48 lipobox sequence is present in most cyanobacteria but is absent in eukaryotes. A possible role for lipoproteins in the coordinated assembly of cyanobacterial PSII is discussed.
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影响因子：
7.4
作者：
Dobakova, Marika;Sobotka, Roman;Komenda, Josef
通讯作者：
Komenda, Josef
DOI：
10.1073/pnas.1323063111
发表时间：
2014-03-25
影响因子：
11.1
作者：
Liu, Haijun;Zhang, Hao;Pakrasi, Himadri B.
通讯作者：
Pakrasi, Himadri B.
影响因子：
4.9
作者：
Knoppova, Jana;Yu, Jianfeng;Komenda, Josef
通讯作者：
Komenda, Josef
影响因子：
4.8
作者：
Kashino, Yasuhiro;Inoue-Kashino, Natsuko;Pakrasi, Himadri B.
通讯作者：
Pakrasi, Himadri B.
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM