Phylogeography and Ecological Niche Modeling Reveal Reduced Genetic Diversity and Colonization Patterns of Skunk Cabbage (Symplocarpus foetidus; Araceae) From Glacial Refugia in Eastern North America.
Phylogeography and Ecological Niche Modeling Reveal Reduced Genetic Diversity and Colonization Patterns of Skunk Cabbage (Symplocarpus foetidus; Araceae) From Glacial Refugia in Eastern North America.
复制标题
系统发育地理学和生态位模型揭示北美东部冰川避难所的臭甘蓝（Symplocarpus foetidus；天南星科）的遗传多样性和定殖模式减少
DOI：
10.3389/fpls.2018.00648
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
Kim SC
中科院分区：
文献类型：
--
作者：
Kim SH;Cho MS;Li P;Kim SC
第四纪冰期和间冰期的交替，极大地影响了整个北方动植物物种的分布和种群遗传结构。令人惊讶的是，很少有人知道冰川后的湿地多年生草本植物，广泛分布在北美东部的落叶林或落叶常绿混合林林下植被的退化历史。在这项研究中，我们调查了臭鼬甘蓝，Symplocarpus foetidus的32个种群之间的种下变异，以测试的假设，即臭鼬甘蓝现存的物种多样性是一个冰后期的范围扩大的结果，从南部避难所在第四纪冰期。从阿巴拉契亚山脉东部和西部的冰川（18个种群，275个人）和未冰川（14个种群，210个人）地区的485个样本中，共4041个碱基对（bp）的叶绿体基因间间隔区（cpDNA）进行测序。计算了单倍型数、单倍型多样性和核苷酸多样性，并通过分子方差分析（AMOVA）评估了群体内和群体间的遗传变异。利用分子方差空间分析（SAMOVA）进一步研究了遗传分化的地理格局。共回收8个单倍型和3个遗传组（SAMOVA）和更高的单倍型数（8个单倍型）和单倍型多样性（0.7425），观察到在未冰期相比，冰期人口（5个单倍型，单倍型多样性= 0.6099）。在冰川地区发现的所有单倍型代表了在非冰川地区发现的单倍型的一个子集。S. foetidus可能在第三纪（中新世中期和上新世晚期），早于末次冰期最大期（LGM）。基于生态位模型（ENM）的预测表明，臭鼬甘蓝在末次盛冰期的适宜栖息地大大减少，栖息地范围进一步向南相比，目前的分布。减少变化和单倍型在冰川地区的子集表明，创始人的影响与范围扩大通过长距离种子传播。我们的研究结果不支持“漂流区”的情况下，北方避难所，而数据表明，“东北”避难所附近的最南端的LGM范围。
Alternating glacial and interglacial periods during the Quaternary have dramatically affected the distribution and population genetic structure of plant and animal species throughout the northern hemisphere. Surprisingly, little is known about the post-glacial recolonization history of wetland herbaceous perennials that are widely distributed in the understory of deciduous or mixed deciduous-evergreen forests in eastern North America. In this study, we investigated infraspecific variation among 32 populations of skunk cabbage, Symplocarpus foetidus, to test the hypothesis that the extant species diversity of skunk cabbage is the result of a post-glacial range expansion from southern refugia during the Quaternary Ice Age. A total of 4041 base pairs (bp) of the chloroplast intergenic spacer region (cpDNA) was sequenced from 485 individuals sampled from glaciated (18 populations, 275 individuals) and unglaciated (14 populations, 210 individuals) regions east and west of the Appalachian Mountains. Haplotype number, haplotype diversity, and nucleotide diversity were calculated, and genetic variation within and among populations was assessed by analysis of molecular variance (AMOVA). The geographic pattern of genetic differentiation was further investigated with a spatial analysis of molecular variance (SAMOVA). A total of eight haplotypes and three genetic groups (SAMOVA) were recovered and a much higher haplotype number (eight haplotypes) and haplotype diversity (0.7425) was observed in unglaciated compared to glaciated populations (five haplotypes, haplotype diversity = 0.6099). All haplotypes found in glaciated regions represented a subset of haplotypes found in unglaciated regions. Haplotypes of S. foetidus likely diverged during the Tertiary (mid-Miocene and late Pliocene), predating the last glacial maximum (LGM). Predictions based on ecological niche modeling (ENM) suggested that there was considerably less suitable habitat for skunk cabbage during the LGM, and the habitat range was further south compared to the current distribution. Reduced variation and a subset of haplotypes in glaciated regions suggest a founder effect associated with range expansion via long-distance seed dispersal. Our results do not support the “Driftless Area” scenario for the northern refugium, rather the data suggest a “Northeastern” refugium near the southernmost extent of the LGM.
登录
查看更多内容
影响因子：
10.7
作者：
Minh BQ;Nguyen MA;von Haeseler A
通讯作者：
von Haeseler A
影响因子：
5.9
作者：
Braunisch, Veronika;Coppes, Joy;Bollmann, Kurt
通讯作者：
Bollmann, Kurt
影响因子：
4.9
作者：
Clement, M;Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
3.1
作者：
Broyles, SB;ShermanBroyles, SL;Rogati, P
通讯作者：
Rogati, P
影响因子：
4.1
作者：
Austin, JD;Lougheed, SC;Boag, PT
通讯作者：
Boag, PT