Cyanovirin-N produced in rice endosperm offers effective pre-exposure prophylaxis against HIV-1BaL infection in vitro.
Cyanovirin-N produced in rice endosperm offers effective pre-exposure prophylaxis against HIV-1BaL infection in vitro.
复制标题
DOI：
10.1007/s00299-016-1963-5
复制
发表时间：
2016-06
影响因子：
6.2
通讯作者：
Capell T
中科院分区：
文献类型：
--
作者：
Vamvaka E;Evans A;Ramessar K;Krumpe LR;Shattock RJ;O'Keefe BR;Christou P;Capell T
Cyanovirin-N (CV-N) is a lectin with potent antiviral activity that has been proposed as a component of microbicides for the prevention of infection with Human immunodeficiency virus (HIV). The production of protein-based microbicide components requires a platform that is sufficiently economical and scalable to meet the demands of the large at-risk population, particularly in resource poor developing countries. We, therefore, expressed CV-N in rice endosperm, because the dried seed is ideal for storage and transport and crude extracts could be prepared locally and used as a microbicide component without further purification. We found that crude extracts from rice seeds expressing up to 10 μg CV-N per gram dry seed weight showed dose-dependent gpl20 binding activity, confirming that the protein was soluble, correctly folded and active. The recombinant lectin (OSCV-N) reduced the infectivity of HIV-lBaL (an R5 virus strain representing the majority of transmitted infections) by ~90 % but showed only weak neutralization activity against HIV-1RF (representative of X4 virus, rarely associated with transmission), suggesting it would be highly effective for pre-exposure prophylaxis against the vast majority of transmitted strains. Crude extracts expressing OSCV-N showed no toxicity towards human cells at working dilutions indicating that microbicide components produced in rice endosperm are safe for direct application as topical microbicides in humans. Cyanovirin-N produced in rice endosperm provides efficient pre-exposure prophylaxis against HIV-lBaL infection in vitro.
登录
查看更多内容
DOI：
10.1126/science.1254031
发表时间：
2014-07-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Carlson JM;Schaefer M;Monaco DC;Batorsky R;Claiborne DT;Prince J;Deymier MJ;Ende ZS;Klatt NR;DeZiel CE;Lin TH;Peng J;Seese AM;Shapiro R;Frater J;Ndung'u T;Tang J;Goepfert P;Gilmour J;Price MA;Kilembe W;Heckerman D;Goulder PJ;Allen TM;Allen S;Hunter E
通讯作者：
Hunter E
影响因子：
7.4
作者：
Grivel JC;Shattock RJ;Margolis LB
通讯作者：
Margolis LB
影响因子：
3.7
作者：
Alexandre, Kabamba B.;Gray, Elin S.;Morris, Lynn
通讯作者：
Morris, Lynn
影响因子：
3.6
作者：
Este, JA;Schols, D;DeClercq, E
通讯作者：
DeClercq, E
DOI：
10.1038/nbt1091-957
发表时间：
1991-10-01
期刊：
BIO-TECHNOLOGY
影响因子：
--
作者：
CHRISTOU, P;FORD, TL;KOFRON, M
通讯作者：
KOFRON, M