Proteomic and bioinformatic profile of primary human oral epithelial cells.
Proteomic and bioinformatic profile of primary human oral epithelial cells.
复制标题
原代人口腔上皮细胞的蛋白质组学和生物信息学特征。
DOI：
10.1021/pr3007254
复制
发表时间：
2012
影响因子：
4.4
通讯作者：
McCormick,ThomasS
中科院分区：
文献类型：
--
作者：
Ghosh,SantoshK;Yohannes,Elizabeth;Bebek,Gurkan;Weinberg,Aaron;Jiang,Bin;Willard,Belinda;Chance,MarkR;Kinter,MichaelT;McCormick,ThomasS
口腔粘膜的创伤经常发生在高度脓毒症的环境中。值得注意的是，这些伤口愈合很快，口腔在很大程度上保持健康。破译正常人口腔上皮细胞（NHOEC）蛋白质组对于理解当粘膜屏障完整时以及当其被破坏时引起的保护机制是至关重要的。结合2D凝胶电泳和鸟枪蛋白质组学鉴定了1662个NHOEC蛋白。蛋白质组注释是基于蛋白质类、分子功能、疾病关联以及典型和代谢信号传导途径中的成员资格进行的。将NHOEC蛋白质组与先天免疫相关相互作用数据库（InnateDB）进行比较，确定了64种与先天免疫相关的常见蛋白质。与已发表的唾液蛋白质组的比较显示，738/1662 NHOEC蛋白是常见的，这表明大量的唾液蛋白是上皮来源的。基因本体分析表明，NHOEC和唾液蛋白质组的生物过程和分子功能的分布相似。我们还评估了NHOEC蛋白质组的个体间变异性，并观察到它与其他原代细胞相当。在这项研究中描述的基线蛋白质组应该作为口腔粘膜蛋白质组研究的资源，特别是在疾病过程中。
Wounding of the oral mucosa occurs frequently in a highly septic environment. Remarkably, these wounds heal quickly and the oral cavity, for the most part, remains healthy. Deciphering the normal human oral epithelial cell (NHOEC) proteome is critical for understanding the mechanism(s) of protection elicited when the mucosal barrier is intact, as well as when it is breached. Combining 2D gel electrophoresis with shotgun proteomics resulted in identification of 1662 NHOEC proteins. Proteome annotations were performed based on protein classes, molecular functions, disease association and membership in canonical and metabolic signaling pathways. Comparing the NHOEC proteome with a database of innate immunity-relevant interactions (InnateDB) identified 64 common proteins associated with innate immunity. Comparison with published salivary proteomes revealed that 738/1662 NHOEC proteins were common, suggesting that significant numbers of salivary proteins are of epithelial origin. Gene ontology analysis showed similarities in the distributions of NHOEC and saliva proteomes with regard to biological processes, and molecular functions. We also assessed the interindividual variability of the NHOEC proteome and observed it to be comparable with other primary cells. The baseline proteome described in this study should serve as a resource for proteome studies of the oral mucosa, especially in relation to disease processes.
登录
查看更多内容
人类血浆蛋白的二维凝胶数据库
影响因子：
2.9
作者：
N. Anderson;N. G. Anderson
通讯作者：
N. G. Anderson
影响因子：
4.4
作者：
Wilmarth, PA;Riviere, MA;David, LL
通讯作者：
David, LL
影响因子：
7
作者：
R. Schwartz;Claire Ting;J. King
通讯作者：
R. Schwartz;Claire Ting;J. King
DOI：
10.1098/rstb.1994.0113
发表时间：
1994-08-30
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HASLETT, C;SAVILL, JS;MEAGHER, LC
通讯作者：
MEAGHER, LC
影响因子：
6.5
作者：
STENN, KS;LINK, R;KUKLINSKA, E
通讯作者：
KUKLINSKA, E