Cell DistensionInduced Increase of the Delayed Rectifier K+ Current in Guinea Pig Ventricular Myocytes
Cell DistensionInduced Increase of the Delayed Rectifier K+ Current in Guinea Pig Ventricular Myocytes
复制标题
细胞膨胀引起豚鼠心室肌细胞延迟整流 K 电流增加
DOI：
10.1161/01.res.78.3.466
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发表时间：
1996
影响因子：
20.1
通讯作者：
A. Noma
中科院分区：
文献类型：
--
作者：
Zhuren Wang;T. Mitsuiye;A. Noma
在全细胞电压箝位过程中，用移液管施加5 ~ 20 mm Hg的正压使豚鼠心脏单心室肌细胞扩张。延迟整流器K +电流的幅值（I K）增加了约1.5倍，而内向整流器K +电流几乎没有受到影响。通过施加−10至−30毫米汞柱的负压，伴随着膨胀细胞的收缩，ik2的增加是可逆的。ik的这种反应很大程度上归因于ik的e -4031不敏感成分。使用长时间去极化脉冲（bbb30秒）测量到+60 mV的完全激活电流振幅，随着细胞膨胀而增加。ik在长脉冲中的激活时间过程由3个以上的指数分量组成，其最慢时间常数由对照的20.2±7.7 s （n=4）减小到7.6±1.6 s （n=5）。我们没有检测到微管或微丝、蛋白激酶C和ca2 +参与通货膨胀介导的ik升高。
Abstract Single ventricular myocytes of guinea pig heart were distended by applying a positive pressure of 5 to 20 mm Hg in the pipette during the whole-cell voltage clamp. The amplitude of delayed rectifier K + current (I K ) was increased by ≈1.5 times, whereas the inward rectifier K + current was scarcely affected. The increase of I K was reversible by applying a negative pressure of −10 to −30 mm Hg accompanied by shrinkage of the inflated cell. This response of I K was largely attributed to the E-4031–insensitive component of I K . The fully activated current amplitude, measured using long-lasting depolarizing pulses (>30 seconds) to +60 mV, was increased by the cell distension. The activation time course of I K during the long pulse consisted of more than three exponential components, and the slowest time constant was decreased by the distension from control 20.2±7.7 seconds (n=4) to 7.6±1.6 seconds (n=5). We failed to detect an involvement of microtubules or microfilaments, protein kinase C, and Ca 2+ in the inflation-mediated increase of I K .
DOI：
10.1113/jphysiol.1993.sp019974
发表时间：
1993-12
期刊：
The Journal of Physiology
影响因子：
--
作者：
Jianping Zhang;R. Rasmusson;S. K. Hall;M. Lieberman
通讯作者：
Jianping Zhang;R. Rasmusson;S. K. Hall;M. Lieberman
影响因子：
3.4
作者：
SOKABE, M;SACHS, F;JING, ZQ
通讯作者：
JING, ZQ
低渗溶液增加爪蟾卵母细胞中表达的缓慢激活的钾电流IsK。
DOI：
10.1016/0006-291x(92)90661-4
发表时间：
1992
影响因子：
3.1
作者：
Busch,AE;Varnum,M;Adelman,JP;North,RA
通讯作者：
North,RA