Horizontal γ-PNA immobilization through organophosphonate chemistry for biosensing applications
Horizontal γ-PNA immobilization through organophosphonate chemistry for biosensing applications
复制标题
通过有机膦酸酯化学水平固定 γ-PNA，用于生物传感应用
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
A. Cattani
中科院分区：
文献类型：
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作者：
A. De;Kevin Keim;M. Tornow;A. Cattani
Silicon-based field effect devices have been widely investigated in recent years for the label-free detection of DNA hybridization. The devices rely on detecting changes in the electrical surface potential that occur as a result of adsorbing charged DNA. To provide surface-immobilized affinity receptors for DNA hybridization, a suitable organic interface is obligatory that has a high density of receptor binding sites and a short distance between surface and probe DNA or its analogue, peptidic nucleic acid (PNA), to minimize electrolyte screening effects. In this work, we report on the bio-functionalization and characterization of silicon oxide-terminated surfaces with γ-PNA through organophosphonate interfacial chemistry. Functionalizing via attachment groups at the γ-points along the PNA backbone allows for multidentate binding of the PNA receptor in a lying configuration on the device surface, with potential application in label-free biosensing device optimization.
DOI：
10.1088/0953-8984/28/9/094008
发表时间：
2016
期刊：
Journal of Physics: Condensed Matter
影响因子：
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作者：
Pathak;Anshuma;Achyut;Kung-Ching;Schmolke;Hannah;Klages;Claus-Peter;Schwartz;Jeffrey;Tornow
通讯作者：
Tornow