Serratia symbiotica Enhances Fatty Acid Metabolism of Pea Aphid to Promote Host Development.
Serratia symbiotica Enhances Fatty Acid Metabolism of Pea Aphid to Promote Host Development.
复制标题
共生沙雷氏菌增强豌豆蚜脂肪酸代谢促进寄主发育。
DOI：
10.3390/ijms22115951
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发表时间：
2021-05-31
影响因子：
5.6
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Zhou X;Ling X;Guo H;Zhu-Salzman K;Ge F;Sun Y
与昆虫相关的细菌共生体经常参与寄主的发育和生态适应。共生沙雷菌（Serratia symbiotica）是豌豆蚜虫体内常见的兼性内共生菌，可提高寄主的适应性和耐热性，但有关携带沙雷菌的蚜虫的营养代谢及其对寄主的影响的研究有限。在本研究中，我们发现感染沙雷氏菌的蚜虫在采食离体叶片时，其若虫发育时间比未感染沙雷氏菌的蚜虫短，体重也比未感染沙雷氏菌的蚜虫高。与脂肪酸生物合成和延伸相关的基因在感染沙雷菌的蚜虫中表达上调。具体而言，脂肪酸合成酶1 （FASN1）和二酰基甘油-o-酰基转移酶2 （DGAT2）的表达升高可导致脂肪体中肉豆蔻酸、棕榈酸、亚油酸和花生酸的积累。通过药理抑制剂或通过抑制FASN1和DGAT2的表达来抑制沙雷菌感染的豌豆蚜虫的脂肪酸合成，延长了若虫的生长期，降低了蚜虫的体重。相反，向这些蚜虫补充肉豆蔻酸（C14:0）可以恢复它们的正常发育和体重增加。结果表明，沙雷菌通过促进脂肪酸的生物合成来促进蚜虫宿主的发育和生长。我们的发现揭示了蚜虫和兼性内共生体共生的营养效应。
Bacterial symbionts associated with insects are often involved in host development and ecological adaptation. Serratia symbiotica, a common facultative endosymbiont harbored in pea aphids, improves host fitness and heat tolerance, but studies concerning the nutritional metabolism and impact on the aphid host associated with carrying Serratia are limited. In the current study, we showed that Serratia-infected aphids had a shorter nymphal developmental time and higher body weight than Serratia-free aphids when fed on detached leaves. Genes connecting to fatty acid biosynthesis and elongation were up-regulated in Serratia-infected aphids. Specifically, elevated expression of fatty acid synthase 1 (FASN1) and diacylglycerol-o-acyltransferase 2 (DGAT2) could result in accumulation of myristic acid, palmitic acid, linoleic acid, and arachidic acid in fat bodies. Impairing fatty acid synthesis in Serratia-infected pea aphids either by a pharmacological inhibitor or through silencing FASN1 and DGAT2 expression prolonged the nymphal growth period and decreased the aphid body weight. Conversely, supplementation of myristic acid (C14:0) to these aphids restored their normal development and weight gain. Our results indicated that Serratia promoted development and growth of its aphid host through enhancing fatty acid biosynthesis. Our discovery has shed more light on nutritional effects underlying the symbiosis between aphids and facultative endosymbionts.
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影响因子：
6.1
作者：
Gao, Xueke;Luo, Junyu;Cui, Jinjie
通讯作者：
Cui, Jinjie
影响因子：
2.2
作者：
Montllor, CB;Maxmen, A;Purcell, AH
通讯作者：
Purcell, AH
影响因子：
3.4
作者：
Li, Chunfen;He, Meng;Peng, Yu
通讯作者：
Peng, Yu
影响因子：
7.7
作者：
Herren JK;Paredes JC;Schüpfer F;Arafah K;Bulet P;Lemaitre B
通讯作者：
Lemaitre B
影响因子：
6.7
作者：
Caragata EP;Rancès E;Hedges LM;Gofton AW;Johnson KN;O'Neill SL;McGraw EA
通讯作者：
McGraw EA